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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2009-6-15
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pubmed:abstractText |
To examine whether grape seed proanthocyanidin extract (GSPE) which is known to act as an antioxidant has therapeutic effect on collagen-induced arthritis (CIA) in mice, an animal model of rheumatoid arthritis. Mice were treated with an intraperitoneal injection of GSPE (10, 50, or 100 mg/kg) or saline. Clinical, histological, and biochemical parameters were assessed. The effects of GSPE on osteoclastogenesis were determined by tartrate-resistant acid phosphatase (TRAP) staining of the inflamed joints and bone-marrow cells cultured with the receptor activator of nuclear factor B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Intracellular levels of hydrogen peroxide were determined using carboxy-dichlorodihydrofluorescein diacetate. GSPE treatment significantly attenuated the severity of CIA in a dose-dependent manner and reduced the histology scores for synovial inflammation, cartilage erosion, bone erosion, and the number of TRAP+ osteoclasts. GSPE treatment significantly reduced the numbers of tumor necrosis factor alpha (TNF-alpha)- or interleukin 17 (IL-17)-producing cells in the synovial tissue and the spontaneous production of TNF-alpha and IL-17 by splenocytes compared with those in the control mice. The serum levels of type-II-collagen-specific IgG2a and plasma levels of 8-isoprostane in the GSPE-treated mice were significantly lower than those in the control mice. GSPE dose-dependently suppressed osteoclastogenesis in vitro. GSPE significantly reduced hydrogen peroxide production by anti-CD3-monoclonal-antibody-stimulated CD4+ splenocytes. These results indicate that intraperitoneal injection of GSPE attenuated CIA in mice. GSPE may be useful in the treatment of rheumatoid arthritis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acid Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Grape Seed Extract,
http://linkedlifedata.com/resource/pubmed/chemical/Grape Seed Proanthocyanidins,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-17,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Isoprostanes,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Colony-Stimulating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Proanthocyanidins,
http://linkedlifedata.com/resource/pubmed/chemical/RANK Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/tartrate-resistant acid phosphatase
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1879-0542
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
4
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pubmed:volume |
124
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
102-10
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:19446580-Acid Phosphatase,
pubmed-meshheading:19446580-Animals,
pubmed-meshheading:19446580-Ankle Joint,
pubmed-meshheading:19446580-Antibodies,
pubmed-meshheading:19446580-Arthritis, Experimental,
pubmed-meshheading:19446580-Arthritis, Rheumatoid,
pubmed-meshheading:19446580-Cells, Cultured,
pubmed-meshheading:19446580-Collagen Type II,
pubmed-meshheading:19446580-Disease Models, Animal,
pubmed-meshheading:19446580-Grape Seed Extract,
pubmed-meshheading:19446580-Hydrogen Peroxide,
pubmed-meshheading:19446580-Interleukin-17,
pubmed-meshheading:19446580-Isoenzymes,
pubmed-meshheading:19446580-Isoprostanes,
pubmed-meshheading:19446580-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:19446580-Mice,
pubmed-meshheading:19446580-Mice, Inbred DBA,
pubmed-meshheading:19446580-Osteoclasts,
pubmed-meshheading:19446580-Plant Extracts,
pubmed-meshheading:19446580-Proanthocyanidins,
pubmed-meshheading:19446580-RANK Ligand,
pubmed-meshheading:19446580-Spleen,
pubmed-meshheading:19446580-Tumor Necrosis Factor-alpha
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pubmed:year |
2009
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pubmed:articleTitle |
Grape seed proanthocyanidin extract (GSPE) attenuates collagen-induced arthritis.
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pubmed:affiliation |
The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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