Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1991-12-24
pubmed:abstractText
Synthetic transcripts of satellite 2 DNA from newts undergo self-catalyzed, site-specific cleavage in vitro. Cleavage occurs within a domain that is similar to the hammerhead domain used by a number of self-cleaving, infectious plant RNAs. The newt hammerhead has a potentially unstable structure due to a stem composed of two base pairs and a 2-nucleotide loop, and unlike other hammerheads that have been studied, it cannot cleave as an isolated unit. Here we show that cleavage by a single newt hammerhead requires additional satellite 2 sequences flanking both ends of the hammerhead domain. We also present a structural model of a truncated satellite 2 transcript which is capable of cleavage. The structure includes an internally looped extension to one of the conserved stems of the hammerhead. By in vitro mutagenesis, the identities of each of the five nucleotides composing one of the internal loops were shown to be critical for cleavage. Additional evidence that the extension stimulates self-cleavage in a manner other than by simply stabilizing the hammerhead is presented.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-1689847, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-1694789, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-1703005, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-1708285, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2020702, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2027778, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2085544, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2107573, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2160856, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2183177, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2185542, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2433049, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2436805, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2441261, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2456468, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2457170, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2474803, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2479828, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2482432, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2583519, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2602373, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2696172, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2740329, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-2762152, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-3184270, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-3323813, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-3594567, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-3771629, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-3891660, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-6163133, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-6198526, http://linkedlifedata.com/resource/pubmed/commentcorrection/1944278-6260957
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6109-15
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Newt satellite 2 transcripts self-cleave by using an extended hammerhead structure.
pubmed:affiliation
Department of Biological Science, Florida State University, Tallahassee 32306-3050.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't