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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2009-7-6
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pubmed:abstractText |
Although green tea polyphenol catechin is considered as a potential anti-inflammatory agent, its effect on bacterial component-induced inflammation has been poorly investigated. We examined the capacity of epigallocatechin gallate (EGCG) to regulate leukocyte responses to bacterial chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (fMLF), which is recognized by a human G protein-coupled receptor FPR on phagocytic leukocytes. Pretreatment of human monocytic cells or FPR-transfected rat basophilic leukemia cells (ETFR cells) with EGCG significantly inhibited fMLF-induced chemotaxis. Intraperitoneal administration of EGCG in mice suppressed fMLF-induced leukocyte infiltration into the air pouch created in the skin. Mechanistic studies revealed that EGCG dose-dependently suppressed fMLF-induced calcium flux in monocytic cells and ETFR cells. fMLF-induced ETFR cell migration was significantly inhibited by a specific MEK1/2 inhibitor, PD98059, which was associated with reduction in fMLF-induced ERK1/2 phosphorylation. These results suggest that EGCG inhibits FPR-mediated leukocyte activation thus is a promising anti-inflammatory compound.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1878-1705
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1126-30
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pubmed:meshHeading |
pubmed-meshheading:19426837-Animals,
pubmed-meshheading:19426837-Antioxidants,
pubmed-meshheading:19426837-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:19426837-Camellia sinensis,
pubmed-meshheading:19426837-Catechin,
pubmed-meshheading:19426837-Cell Line, Tumor,
pubmed-meshheading:19426837-Cell Movement,
pubmed-meshheading:19426837-Flavonoids,
pubmed-meshheading:19426837-Humans,
pubmed-meshheading:19426837-Inflammation,
pubmed-meshheading:19426837-Injections, Intraperitoneal,
pubmed-meshheading:19426837-Leukemia, Basophilic, Acute,
pubmed-meshheading:19426837-Mice,
pubmed-meshheading:19426837-Monocytes,
pubmed-meshheading:19426837-N-Formylmethionine Leucyl-Phenylalanine,
pubmed-meshheading:19426837-Rats,
pubmed-meshheading:19426837-Receptors, Formyl Peptide,
pubmed-meshheading:19426837-Signal Transduction,
pubmed-meshheading:19426837-Transfection,
pubmed-meshheading:19426837-Transgenes
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pubmed:year |
2009
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pubmed:articleTitle |
The green tea polyphenol (-)-epigallocatechin-3-gallate inhibits leukocyte activation by bacterial formylpeptide through the receptor FPR.
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pubmed:affiliation |
Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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