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pubmed-article:19404618pubmed:abstractTextThiacalix[4]arenetetrasulfonate was treated with Ce(IV) in water at pH 9.5 to give novel phosphoester-hydrolyzing complexes. The dinuclear Ce(IV) complex promoted the hydrolysis of p-nitrophenyl phosphate with a turnover frequency of 6.8 h(-1) at 50 degrees C, showing fourfold higher activity than the mononuclear complex. The dinuclear complex was readily immobilized onto an antibody by simply mixing them in water, hence its phosphatase-like activity was applied to the color-developing reaction in immunoassay. The model assay using an antibody labeled with the dinuclear complex allowed the detection of as little as 10 ng mL(-1) of a tumor marker, Bence-Jones protein, in a 96-well microtiter plate format. Analysis of urine for Bence-Jones protein was performed by the proposed method.lld:pubmed
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pubmed-article:19404618pubmed:year2009lld:pubmed
pubmed-article:19404618pubmed:articleTitlePhosphoester hydrolysis by cerium(IV)-thiacalix[4]arene complexes and its application to immunoassay.lld:pubmed
pubmed-article:19404618pubmed:affiliationDepartment of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. h-matsu@numse.nagoya-u.ac.jplld:pubmed
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pubmed-article:19404618pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed