Linked Life Data

19404618

Source:http://linkedlifedata.com/resource/pubmed/id/19404618

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-6-11
pubmed:abstractText
Thiacalix[4]arenetetrasulfonate was treated with Ce(IV) in water at pH 9.5 to give novel phosphoester-hydrolyzing complexes. The dinuclear Ce(IV) complex promoted the hydrolysis of p-nitrophenyl phosphate with a turnover frequency of 6.8 h(-1) at 50 degrees C, showing fourfold higher activity than the mononuclear complex. The dinuclear complex was readily immobilized onto an antibody by simply mixing them in water, hence its phosphatase-like activity was applied to the color-developing reaction in immunoassay. The model assay using an antibody labeled with the dinuclear complex allowed the detection of as little as 10 ng mL(-1) of a tumor marker, Bence-Jones protein, in a 96-well microtiter plate format. Analysis of urine for Bence-Jones protein was performed by the proposed method.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1618-2650
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
394
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1471-6
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Phosphoester hydrolysis by cerium(IV)-thiacalix[4]arene complexes and its application to immunoassay.
pubmed:affiliation
Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. h-matsu@numse.nagoya-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't