|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
11
|
|
pubmed:dateCreated |
1991-12-16
|
|
pubmed:abstractText |
The yeast HO gene is transcribed transiently during G1 as cells undergo START. START-specific HO activation requires two proteins, SWI4 and SWI6, which act via a motif (CACGA4) repeated up to 10 times within the URS2 region of the HO promoter. We identified a DNA-binding activity containing SWI4 and SWI6 that recognizes the CACGA4 sequences within URS2. Two forms of SWI4,6-DNA complexes called L and U can be distinguished by their electrophoretic mobility. L complexes can be detected at all stages of the cell cycle, but U complexes are only detected in cells that have undergone START. The formation of U complexes may be the trigger of HO activation. The SWI6 protein is concentrated in the nucleus throughout G1, but at some point in S or G2 significant amounts accumulate in the cytoplasm. This change in cellular location of the SWI6 protein might contribute to the turnoff of HO transcription after cells have undergone START.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleases, Type II...,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HO protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/SCEI protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/SWI4 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0890-9369
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
5
|
|
pubmed:geneSymbol |
HO
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2000-13
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:1936990-Base Sequence,
pubmed-meshheading:1936990-Cell Cycle,
pubmed-meshheading:1936990-DNA, Fungal,
pubmed-meshheading:1936990-DNA-Binding Proteins,
pubmed-meshheading:1936990-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:1936990-Fungal Proteins,
pubmed-meshheading:1936990-Gene Expression Regulation, Fungal,
pubmed-meshheading:1936990-Genes, Fungal,
pubmed-meshheading:1936990-Kinetics,
pubmed-meshheading:1936990-Molecular Sequence Data,
pubmed-meshheading:1936990-Precipitin Tests,
pubmed-meshheading:1936990-Promoter Regions, Genetic,
pubmed-meshheading:1936990-Protein Kinases,
pubmed-meshheading:1936990-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:1936990-Saccharomyces cerevisiae,
pubmed-meshheading:1936990-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:1936990-Transcription Factors,
pubmed-meshheading:1936990-Transcriptional Activation
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Changes in a SWI4,6-DNA-binding complex occur at the time of HO gene activation in yeast.
|
|
pubmed:affiliation |
Research Institute of Molecular Pathology, Vienna, Austria.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
