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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2009-4-10
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pubmed:abstractText |
Genotypic surveys suggest that human immunodeficiency virus type 1 (HIV-1) and HIV-2 evolve different sets of mutations in response to nucleoside reverse-transcriptase inhibitors (NRTIs). We used site-directed mutagenesis, culture-based phenotyping, and cell-free assays to determine the resistance profiles conferred by specific amino acid replacements in HIV-2 reverse transcriptase. Although thymidine analogue mutations had no effect on zidovudine sensitivity, the addition of Q151M together with K65R or M184V was sufficient for high-level resistance to both lamivudine and zidovudine in HIV-2, and the combination of K65R, Q151M, and M184V conferred classwide NRTI resistance. These data suggest that current NRTI-based regimens are suboptimal for treating HIV-2 infection.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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|
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1899
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
199
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1323-6
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pubmed:meshHeading |
pubmed-meshheading:19358668-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:19358668-Africa, Western,
pubmed-meshheading:19358668-Amino Acid Substitution,
pubmed-meshheading:19358668-Anti-HIV Agents,
pubmed-meshheading:19358668-Antiviral Agents,
pubmed-meshheading:19358668-Drug Resistance, Viral,
pubmed-meshheading:19358668-Genetic Predisposition to Disease,
pubmed-meshheading:19358668-HIV Infections,
pubmed-meshheading:19358668-HIV-1,
pubmed-meshheading:19358668-HIV-2,
pubmed-meshheading:19358668-Humans,
pubmed-meshheading:19358668-Mutagenesis, Site-Directed,
pubmed-meshheading:19358668-Phenotype,
pubmed-meshheading:19358668-RNA-Directed DNA Polymerase,
pubmed-meshheading:19358668-Reverse Transcriptase Inhibitors,
pubmed-meshheading:19358668-Zidovudine
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pubmed:year |
2009
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pubmed:articleTitle |
Antiretroviral drug resistance in HIV-2: three amino acid changes are sufficient for classwide nucleoside analogue resistance.
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pubmed:affiliation |
Department of Pathology, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA 98133, USA. smithra@u.washington.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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