Linked Life Data

19347320

Source:http://linkedlifedata.com/resource/pubmed/id/19347320

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-4-6
pubmed:abstractText
Macrophages are not only host cells for many pathogens, but also fulfill several key functions in the innate and adaptive immune response, including the release of pro- and anti-inflammatory cytokines, the generation of organic and inorganic autacoids, the phagocytosis and killing of intracellular microorganisms or tumor cells, and the degradation and presentation of antigens. Several of these functions are shared by other immune cells, including dendritic cells, granulocytes, NK cells, and/or T lymphocytes. Thus, the analysis of macrophage functions in vitro using primary mouse cell populations requires standardized methods for the generation and culture of macrophages that guarantee high cell purity as well as the absence of stimulatory microbial contaminants. This chapter presents methodology to achieve these aims.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
531
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
203-24
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Generation, culture and flow-cytometric characterization of primary mouse macrophages.
pubmed:affiliation
Institute of Clinical Microbiology, Immunology and Hygiene, University Clinic of Erlangen, Erlangen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't