Linked Life Data

19345674

Source:http://linkedlifedata.com/resource/pubmed/id/19345674

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-4-29
pubmed:abstractText
The presence of the cholesterol ozonolysis products, 3beta-hydroxy-5-oxo-5,6-secocholestan-6-al (atheronal-A) and its aldolization product 3beta-hydroxy-5beta-hydroxy-B-norcholestane-6beta-carboxaldehyde (atheronal-B) in human atherosclerotic tissues was recently reported as evidence for the generation of ozone by activated human neutrophils. However, the mechanism for the formation of atheronals in atherosclerotic tissues is unknown. In this study, we found that atheronals were formed by the reaction of cholesterol with human myeloperoxidase (MPO) in the presence of its substrates H(2)O(2) and Cl(-). The omission of either H(2)O(2) or Cl(-) from the MPO-H(2)O(2)-Cl(-) system resulted in a significant reduction in yields. The formation of atheronals by the MPO-H(2)O(2)-Cl(-) system was inhibited by an inhibitor of MPO and scavengers of reactive oxygen species such as sodium azide, methionine, beta-carotene, and vinylbenzoic acid. Our results suggest that MPO produces atheronals at least partly through an ozone-free mechanism, via the reaction of cholesterol with singlet oxygen generated from HOCl and H(2)O(2).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1090-2104
pubmed:author
pubmed:issnType
Electronic
pubmed:day
29
pubmed:volume
383
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
222-7
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Formation of cholesterol ozonolysis products through an ozone-free mechanism mediated by the myeloperoxidase-H2O2-chloride system.
pubmed:affiliation
Laboratory of Biochemistry and Global Center of Excellence Program, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't