Linked Life Data

19336887

Source:http://linkedlifedata.com/resource/pubmed/id/19336887

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-4-1
pubmed:abstractText
This study was designed to investigate whether hydroxytyrosol (HT) may ameliorate oxidative stress and nuclear factor kappaB (NF-kappaB) activation in the lipopolysaccharide (LPS)-stimulated THP-1 cell line. We measured the intracellular reactive oxygen species (ROS) formation using 2,7-dichlorofluorescein diacetate (DCFH-DA) as a fluorescent probe. Intracellular glutathione (GSH) level was estimated by fluorometric methods. Nitric oxide (NO) production was measured as nitrite (a stable metabolite of NO) concentrations using the Griess reagent system following Jiancheng Institute of Biotechnology protocols. To study the effect of HT on LPS-induced NF-kappaB activation in THP-1 cells, Western blot analysis of the nuclear fraction of cell lysates was performed. The results showed that treatment of THP-1 cells with HT significantly reduced LPS-stimulated NO production and ROS formation in a concentration-dependent manner. HT at 50 and 100 microM concentrations increased the GSH level. The specific DNA-binding activities of NF-kappaB on nuclear extracts from 50 and 100 microM HT treatments were significantly suppressed. The antioxidant N-acetylcysteine (NAC) also showed the same effects as HT on LPS-induced ROS and NO generation, change of GSH level, and NF-kappaB activation. These findings suggest that HT has antioxidant activity to suppress intracellular oxidative stress and NF-kappaB activation in THP-1 cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0918-6158
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
578-82
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Suppressive effects of hydroxytyrosol on oxidative stress and nuclear Factor-kappaB activation in THP-1 cells.
pubmed:affiliation
Department of Laboratory Medicine, College of Medicine, Dalian University, China.
pubmed:publicationType
Journal Article