Linked Life Data

19334785

Source:http://linkedlifedata.com/resource/pubmed/id/19334785

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2009-5-6
pubmed:abstractText
A sensitive chemiluminescence method based on a luminol-myoglobin system is proposed for the determination of melamine in milk products. It was found that the mixed solutions of melamine and myoglobin could react to form a complex on line, which could greatly inhibit the chemiluminescence intensity generated from the reaction between luminol and myoglobin. The decrease in chemiluminescence intensity was proportional to the concentration of melamine, giving a calibration graph linear over the concentration from 10 pg mL(-1) to 50 ng mL(-1) (R(2) = 0.9988) with a detection limit of 3 pg mL(-1) (3sigma). At a flow rate of 2.0 mL min(-1), one analysis cycle, including sampling and washing, could be accomplished in 20 s with a relative standard deviation of <4.0%. The proposed method was applied successfully to the determination of melamine in milk products, and the recovery was from 93.4 to 106.5%. The possible mechanism of luminol-myoglobin-melamine reaction is given.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1520-5118
pubmed:author
pubmed:issnType
Electronic
pubmed:day
13
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3464-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Subpicogram determination of melamine in milk products using a luminol-myoglobin chemiluminescence system.
pubmed:affiliation
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Material Science, Northwest University, Xi'an 710069, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't