Linked Life Data

19305999

Source:http://linkedlifedata.com/resource/pubmed/id/19305999

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-6-8
pubmed:abstractText
The vast majority of microorganisms live under starvation-associated stress conditions that cause mutagenesis despite the limitation of DNA replication and cell division. In this study, we compared the roles of polymerase zeta (Pol zeta) and non-homologous DNA-end joining (NHEJ) in starvation-associated spontaneous base substitutions and frameshifts, using yeast mutants carrying deletions of REV3 (encoding the catalytic subunit of Pol zeta), YKU80 (encoding a protein involved in the initiation of NHEJ), or both genes. We found that approximately 50% of starvation-associated spontaneous frameshifts and 40% of base substitutions required NHEJ to occur. The role of Pol zeta was only slightly less pronounced, with 30-40% of frameshifts and 35-45% of base substitutions being dependent on Rev3. In comparison with the single mutants, the rev3 yku80 double mutant showed an additive decrease in the level of both base substitutions and frameshifts, indicating that Pol zeta and NHEJ function independently in starvation-associated mutagenesis. Our results also imply that about 30% of starvation-associated base substitutions and frameshifts arise by some unknown mechanism that does not involve Pol zeta or NHEJ.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1432-0983
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-51
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Evaluation of the roles of Pol zeta and NHEJ in starvation-associated spontaneous mutagenesis in the yeast Saccharomyces cerevisiae.
pubmed:affiliation
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't