Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-4-27
pubmed:abstractText
Antibiotic resistance determination of Ureaplasma spp. (Ureaplasma parvum and Ureaplasma urealyticum) usually requires predetermination of bacterial titer, followed by antibiotic interrogation using a set bacterial input. This 96-well method allows simultaneous quantification of bacteria in the presence and absence of antibiotics. A method for determining precise MICs and a method for screening against multiple antibiotics using breakpoint thresholds are detailed. Of the 61 Ureaplasma-positive clinical isolates screened, one (1.6%) was resistant to erythromycin (MIC, >64 mg/liter) and clarithromycin (MIC, 4 mg/liter), one to ciprofloxacin (1.6%), and one to tetracycline/doxycycline (1.6%). Five isolates were also consistently found to have an elevated MIC of 8 mg/liter for erythromycin, but this may not represent true antibiotic resistance, as no mutations were found in the 23S rRNA operons or ribosome-associated L4 and L22 proteins for these strains. However, two amino acids (R66Q67) were deleted from the L4 protein of the erythromycin-/clarithromycin-resistant strain. The tetM genetic element was detected in the tetracycline-resistant clinical isolate as well as in the positive control Vancouver strain serotype 9. The tetM gene was also found in a fully tetracycline-susceptible Ureaplasma clinical isolate, and no mutations were found in the coding region that would explain its failure to mediate tetracycline resistance. An amino acid substitution (D82N) was found in the ParC subunit of the ciprofloxacin-resistant isolate, adjacent to the S83L mutation reported by other investigators in many ciprofloxacin-resistant Ureaplasma isolates. It is now possible to detect antibiotic resistance in Ureaplasma within 48 h of positive culture without prior knowledge of bacterial load, identifying them for further molecular analysis.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-10898684, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-10952617, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-10968119, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-11048724, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-11083638, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-11083646, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-12462154, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-13138817, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-14506049, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-1526460, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-1549404, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-1553356, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-16223956, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-16260207, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-16369542, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-16371861, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-16597903, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-16645486, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-16734777, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-16802622, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-17196370, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-17317421, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-18025113, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-18174297, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-18707551, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-2767221, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-3344217, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-3360691, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-3521481, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-3540907, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-7608879, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-7928988, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-8077390, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-8399906, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-8399918, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-8399919, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-9278055, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-9334863, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-9593171, http://linkedlifedata.com/resource/pubmed/commentcorrection/19273669-9986808
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1098-6596
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2020-7
pubmed:dateRevised
2010-9-22
pubmed:meshHeading
pubmed-meshheading:19273669-Humans, pubmed-meshheading:19273669-Anti-Bacterial Agents, pubmed-meshheading:19273669-Infant, Newborn, pubmed-meshheading:19273669-Species Specificity, pubmed-meshheading:19273669-Culture Media, pubmed-meshheading:19273669-Erythromycin, pubmed-meshheading:19273669-Tetracycline, pubmed-meshheading:19273669-Infant, Premature, Diseases, pubmed-meshheading:19273669-Bacterial Proteins, pubmed-meshheading:19273669-Microbial Sensitivity Tests, pubmed-meshheading:19273669-Drug Resistance, Bacterial, pubmed-meshheading:19273669-Bronchoalveolar Lavage Fluid, pubmed-meshheading:19273669-Sequence Analysis, DNA, pubmed-meshheading:19273669-Ureaplasma, pubmed-meshheading:19273669-Point Mutation, pubmed-meshheading:19273669-Ciprofloxacin, pubmed-meshheading:19273669-Ureaplasma Infections, pubmed-meshheading:19273669-Clarithromycin
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