Linked Life Data

19270370

Source:http://linkedlifedata.com/resource/pubmed/id/19270370

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-3-23
pubmed:abstractText
Nocardioides sp. DF412 degrades dibenzofuran (DF) to salicylate through the sequential actions of DF dioxygenase (dfdA), extradiol dioxygenase (dfdB), and hydrolase (dfdC). The involvement of a TetR-type regulator gene dfdS in the dfdB and dfdS gene expression was investigated. A reporter assay using a luciferase gene indicated repression of dfdB and dfdS promoter activities in the presence of the dfdS gene product, DfdS. Gel shift analysis indicated specific binding of DfdS to the dfdB promoter region. Both the presence of a DF-degradation intermediate, 2,2',3-trihydroxybiphenyl (2,2',3-THBP) or its analog, 2,3-dihydroxybiphenyl (2,3-DHBP), and mutation in the inverted repeat (IR) in the dfdB promoter, canceled repression by DfdS in vivo and the binding of DfdS to the dfdB promoter fragment in vitro. These results suggest derepression of DfdS in the presence of 2,2',3-THBP and 2,3-DHBP and the involvement of the IR in the repression by DfdS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1347-6947
pubmed:author
pubmed:issnType
Electronic
pubmed:day
23
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
508-16
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Characterization of a transcriptional regulatory gene involved in dibenzofuran degradation by Nocardioides sp. strain DF412.
pubmed:affiliation
Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't