Linked Life Data

19236908

Source:http://linkedlifedata.com/resource/pubmed/id/19236908

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-4-14
pubmed:abstractText
The spinothalamic tract (STT) neurons in the spinal dorsal horn play an important role in transmission and processing of nociceptive sensory information. Although transient receptor potential vanilloid type 1 (TRPV1) receptors are present in the spinal cord dorsal horn, their physiological function is not fully elucidated. In this study, we examined the role of TRPV1 in modulating neuronal activity of the STT neurons through excitatory and inhibitory synaptic inputs. Whole-cell patch-clamp recordings were performed on STT neurons labeled by a retrograde fluorescent tracer injected into the ventral posterior lateral (VPL) nucleus of the thalamus. Capsaicin (1 microM) increased the frequency of miniature excitatory postsynaptic currents (mEPSC) without changing the amplitude or decay time constant of mEPSC. In contrast, capsaicin had no distinct effect on GABAergic miniature inhibitory postsynaptic currents (mIPSC). Capsazepine (10 microM), a TRPV1 receptor antagonist, abolished the effect of capsaicin on mEPSCs. Capsazepine itself did not affect the baseline amplitude and frequency of mEPSC. The effect of capsaicin on mEPSC was also abolished by removal of external Ca(2+), but not by treatment with Cd(2+). Furthermore, capsaicin increased the firing activity of the STT neurons and this increase in neuronal activity by capsaicin was abolished in the presence of non-N-methyl-d-aspartic acid (NMDA) and NMDA receptor antagonists, 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX) and (R)-amino-5-phosphonovaleric acid (APV). These data suggest that activation of TRPV1 potentiates the glutamate release from excitatory terminals of primary afferent fibers and subsequently increases the neural activity of STT neurons of the rat spinal cord deep dorsal horn.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1873-7544
pubmed:author
pubmed:issnType
Electronic
pubmed:day
5
pubmed:volume
160
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
508-16
pubmed:meshHeading
pubmed-meshheading:19236908-Animals, pubmed-meshheading:19236908-Calcium Channels, pubmed-meshheading:19236908-Capsaicin, pubmed-meshheading:19236908-Excitatory Postsynaptic Potentials, pubmed-meshheading:19236908-Female, pubmed-meshheading:19236908-Glutamic Acid, pubmed-meshheading:19236908-Male, pubmed-meshheading:19236908-Miniature Postsynaptic Potentials, pubmed-meshheading:19236908-Pain, pubmed-meshheading:19236908-Posterior Horn Cells, pubmed-meshheading:19236908-Rats, pubmed-meshheading:19236908-Rats, Sprague-Dawley, pubmed-meshheading:19236908-Sensory System Agents, pubmed-meshheading:19236908-Spinothalamic Tracts, pubmed-meshheading:19236908-Statistics, Nonparametric, pubmed-meshheading:19236908-Synaptic Transmission, pubmed-meshheading:19236908-TRPV Cation Channels, pubmed-meshheading:19236908-gamma-Aminobutyric Acid
pubmed:year
2009
pubmed:articleTitle
Transient receptor potential vanilloid type 1 receptor regulates glutamatergic synaptic inputs to the spinothalamic tract neurons of the spinal cord deep dorsal horn.
pubmed:affiliation
Department of Physiology, Seoul National University College of Medicine, 28 Yungundong Jongnogu, Seoul 110-799, Korea.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't