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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2009-4-7
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pubmed:databankReference |
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pubmed:abstractText |
The effects of silicic acid on the growth of Thermus thermophilus TMY, an extreme thermophile isolated from a siliceous deposit formed from geothermal water at a geothermal power plant in Japan, were examined at 75 degrees C. At concentrations higher than the solubility of amorphous silica (400 to 700 ppm SiO(2)), a silica-induced protein (Sip) was isolated from the cell envelope fraction of log-phase TMY cells grown in the presence of supersaturated silicic acid. Two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the molecular mass and pI of Sip to be about 35 kDa and 9.5, respectively. Induction of Sip expression occurred within 1 h after the addition of a supersaturating concentration of silicic acid to TM broth. Expression of Sip-like proteins was also observed in other thermophiles, including T. thermophilus HB8 and Thermus aquaticus YT-1. The amino acid sequence of Sip was similar to that of the predicted solute-binding protein of the Fe(3+) ABC transporter in T. thermophilus HB8 (locus tag, TTHA1628; GenBank accession no. NC_006461; GeneID, 3169376). The sip gene (987-bp) product showed 87% identity with the TTHA1628 product and the presumed Fe(3+)-binding protein of T. thermophilus HB27 (locus tag TTC1264; GenBank accession no. NC_005835; GeneID, 2774619). Within the genome, sip is situated as a component of the Fbp-type ABC transporter operon, which contains a palindromic structure immediately downstream of sip. This structure is conserved in other T. thermophilus genomes and may function as a terminator that causes definitive Sip expression in response to silica stress.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-10550045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-12449852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-12664138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-14526004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-15064768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-15643937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-15666688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-17216354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-17496104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-17850299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-17993517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-18023417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-18781887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-2404942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-3131301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-3957870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-7559648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-8606197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-9244288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19233950-9892638
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1098-5336
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2406-13
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:19233950-Amino Acid Sequence,
pubmed-meshheading:19233950-Bacterial Proteins,
pubmed-meshheading:19233950-DNA, Bacterial,
pubmed-meshheading:19233950-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:19233950-Geologic Sediments,
pubmed-meshheading:19233950-Hot Springs,
pubmed-meshheading:19233950-Japan,
pubmed-meshheading:19233950-Molecular Sequence Data,
pubmed-meshheading:19233950-Molecular Weight,
pubmed-meshheading:19233950-Phylogeny,
pubmed-meshheading:19233950-Proteome,
pubmed-meshheading:19233950-Sequence Analysis, DNA,
pubmed-meshheading:19233950-Sequence Homology, Amino Acid,
pubmed-meshheading:19233950-Silicic Acid,
pubmed-meshheading:19233950-Thermus thermophilus
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pubmed:year |
2009
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pubmed:articleTitle |
Stimulation of expression of a silica-induced protein (Sip) in Thermus thermophilus by supersaturated silicic acid.
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pubmed:affiliation |
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. doi@agr.kyushu-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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