Source:http://linkedlifedata.com/resource/pubmed/id/19232866
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2009-3-23
|
| pubmed:abstractText |
Diagnosing dengue in febrile patients is challenging. Of a total of 459 patients with acute undifferentiated fever, randomly selected from 12 primary health facilities and 1 clinic of the provincial malaria station in southern Vietnam, dengue-specific antibody (Ab) and NS1Ag enzyme-linked immunosorbent assay (ELISA) (Platelia, Bio-Rad Laboratories, Hercules, CA 94547, US) were performed on acute (t0) and convalescent (t3 weeks) sera. Polymerase chain reaction (PCR) was used for confirmation. Based on a composite of the NS1Ag-ELISA, Ab-ELISA, and PCR results, 54 (12%) patients had acute dengue. Positive and negative predictive values were 65% and 98% for the Ab-based diagnosis and 91% and 92% for NS1Ag, respectively. The agreement between Ab- and NS1Ag-based diagnosis was poor (kappa value, 0.2). Two patients without dengue had detectable NS1Ag on t0 and t3, 1 just above the cutoff value and 1 with very high values. For 5 dengue patients, NS1Ag was still detectable at very high levels at t3. Dengue NS1Ag can be used for early diagnosis of dengue; infrequent false-positive results need further clarification.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/NS1 protein, Dengue virus type 2,
http://linkedlifedata.com/resource/pubmed/chemical/NS1 protein, dengue-1 virus,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Nonstructural Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
1879-0070
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| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:volume |
63
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
372-8
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| pubmed:meshHeading |
pubmed-meshheading:19232866-Adolescent,
pubmed-meshheading:19232866-Adult,
pubmed-meshheading:19232866-Antibodies, Viral,
pubmed-meshheading:19232866-Child,
pubmed-meshheading:19232866-Dengue,
pubmed-meshheading:19232866-Dengue Virus,
pubmed-meshheading:19232866-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:19232866-Female,
pubmed-meshheading:19232866-Fever of Unknown Origin,
pubmed-meshheading:19232866-Humans,
pubmed-meshheading:19232866-Male,
pubmed-meshheading:19232866-Predictive Value of Tests,
pubmed-meshheading:19232866-RNA, Viral,
pubmed-meshheading:19232866-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19232866-Statistics as Topic,
pubmed-meshheading:19232866-Vietnam,
pubmed-meshheading:19232866-Viral Nonstructural Proteins,
pubmed-meshheading:19232866-Young Adult
|
| pubmed:year |
2009
|
| pubmed:articleTitle |
Detection of dengue nonstructural 1 (NS1) protein in Vietnamese patients with fever.
|
| pubmed:affiliation |
Division of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Center, Amsterdam, The Netherlands.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|