Source:http://linkedlifedata.com/resource/pubmed/id/19220857
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2009-3-9
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pubmed:abstractText |
The binding sequences for signal transducer and activator of transcription (STAT) and activator protein 1 have been found in the promoter region of the angiotensinogen gene. We examined whether the elements for activator protein 1 and STAT5B function in angiotensinogen gene activation induced by angiotensin II in vascular smooth muscle cells. Stimulation with angiotensin II increased the level of angiotensinogen mRNA by 2.1-fold in vascular smooth muscle cells. The increased level of angiotensinogen mRNA occurred with concurrent elevations in the levels of STAT5B and c-Jun phosphorylation after stimulation with angiotensin II. Likewise, angiotensin II resulted in similar enhancements of the DNA-binding activity of STAT5B and c-Jun in angiotensin II-induced angiotensinogen expression. Notably, the STAT5B-DNA complex interacted with the c-Jun-DNA complex by forming a stable quaternary complex in angiotensin II-induced angiotensinogen expression. Our findings support a model in which co-operative interaction of STAT5B and activator protein 1 bound to the the promoter region provides maximal activation of angiotensinogen expression by angiotensin II in vascular smooth muscle cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensinogen,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/STAT5 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1742-4658
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1720-8
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pubmed:meshHeading |
pubmed-meshheading:19220857-Angiotensin II,
pubmed-meshheading:19220857-Angiotensinogen,
pubmed-meshheading:19220857-Base Sequence,
pubmed-meshheading:19220857-Cell Line,
pubmed-meshheading:19220857-Chromatin Immunoprecipitation,
pubmed-meshheading:19220857-DNA Primers,
pubmed-meshheading:19220857-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:19220857-Gene Expression Regulation,
pubmed-meshheading:19220857-Humans,
pubmed-meshheading:19220857-Muscle, Smooth, Vascular,
pubmed-meshheading:19220857-Promoter Regions, Genetic,
pubmed-meshheading:19220857-RNA, Messenger,
pubmed-meshheading:19220857-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19220857-STAT5 Transcription Factor,
pubmed-meshheading:19220857-Transcription Factor AP-1
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pubmed:year |
2009
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pubmed:articleTitle |
Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Hebei Medical University, Shijiazhuang, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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