|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2010-6-3
|
|
pubmed:abstractText |
Hepatitis C virus (HCV) is a serious global health threat and current medical treatment options are limited. Interferon (IFN)-gamma is an important proinflammatory cytokine with antiviral activity. However, the mechanism of IFN-gamma in anti-HCV infection remains unclear. In this study, we investigated the role of IFN-gamma on HCV infection of polarized Caco-2 cells using cell culture-derived HCV (HCVcc). We found that downregulation of claudin-1 (CLDN1) induced by IFN-gamma resulted in disruption of barrier function as demonstrated by measurement of transepithelial electrical resistance and dextran permeability. Further, results from confocal microscopy and Western blot analysis showed that in addition to the reduction of CLDN1 expression, IFN-gamma treatment also led to significant changes in the distribution of CLDN1, CD81, and scavenger receptor class B type I. Moreover, infection assays revealed that IFN-gamma-treated cells showed decreased susceptibility to HCVcc infection. These results suggest a novel mechanism that IFN-gamma may inhibit HCV infection by regulating CLDN1 expression and distribution of HCV receptors.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD81,
http://linkedlifedata.com/resource/pubmed/chemical/Antiviral Agents,
http://linkedlifedata.com/resource/pubmed/chemical/CD81 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SCARB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Scavenger Receptors, Class B,
http://linkedlifedata.com/resource/pubmed/chemical/claudin 1
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
1557-7465
|
|
pubmed:author |
pubmed-author:BøeH AHA,
pubmed-author:BaiXue-FanXF,
pubmed-author:JiaZhan-ShengZS,
pubmed-author:LIMR KRK,
pubmed-author:LianJian-QiJQ,
pubmed-author:MaLiL,
pubmed-author:MeeSS,
pubmed-author:WangJiu-PingJP,
pubmed-author:WangPing-ZhongPZ,
pubmed-author:YeLingL,
pubmed-author:ZhangYeY
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
29
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
171-8
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:19196072-Antigens, CD,
pubmed-meshheading:19196072-Antigens, CD81,
pubmed-meshheading:19196072-Antiviral Agents,
pubmed-meshheading:19196072-Blotting, Western,
pubmed-meshheading:19196072-Caco-2 Cells,
pubmed-meshheading:19196072-Cell Line, Tumor,
pubmed-meshheading:19196072-Down-Regulation,
pubmed-meshheading:19196072-Fluorescent Antibody Technique,
pubmed-meshheading:19196072-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:19196072-Hepacivirus,
pubmed-meshheading:19196072-Humans,
pubmed-meshheading:19196072-Interferon-gamma,
pubmed-meshheading:19196072-Membrane Proteins,
pubmed-meshheading:19196072-Microscopy, Confocal,
pubmed-meshheading:19196072-RNA Interference,
pubmed-meshheading:19196072-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19196072-Scavenger Receptors, Class B,
pubmed-meshheading:19196072-Time Factors,
pubmed-meshheading:19196072-Virus Replication
|
|
pubmed:year |
2009
|
|
pubmed:articleTitle |
Inhibition of hepatitis C virus infection by interferon-gamma through downregulating claudin-1.
|
|
pubmed:affiliation |
Center of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
