Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
2009-2-5
pubmed:abstractText
Human cytosolic phenylalanyl-tRNA synthetase (hcPheRS) is responsible for the covalent attachment of phenylalanine to its cognate tRNA(Phe). Significant differences between the amino-acid sequences of eukaryotic and prokaryotic PheRSs indicate that the domain composition of hcPheRS differs from that of the Thermus thermophilus analogue. As a consequence of the absence of the anticodon-recognizing B8 domain, the binding mode of tRNA(Phe) to hcPheRS is expected to differ from that in prokaryotes. Recombinant hcPheRS protein was purified to homogeneity and crystallized. The crystals used for structure determination diffracted to 3.3 A resolution and belonged to space group C2, with unit-cell parameters a = 362.9, b = 213.6, c = 212.7 A, beta = 125.2 degrees . The structure of hcPheRS was determined by the molecular-replacement method in combination with phase information from multiwavelength anomalous dispersion.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-10049785, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-10089360, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-10966471, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-11567147, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-11858721, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-12930986, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-17003130, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-17164524, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-18611382, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-2031086, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-5700707, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-6369319, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-7664121, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-7678431, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-9016717, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-9177188, http://linkedlifedata.com/resource/pubmed/commentcorrection/19193993-9757107
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1744-3091
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-7
pubmed:dateRevised
2011-7-22
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Crystallization and X-ray analysis of human cytoplasmic phenylalanyl-tRNA synthetase.
pubmed:affiliation
Department of Structural Biology, Weizmann Institute of Science, 76100 Rehovot, Israel.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't