Linked Life Data

19192390

Source:http://linkedlifedata.com/resource/pubmed/id/19192390

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-2-4
pubmed:abstractText
To understand the molecular mechanism underlying proline accumulation in Brassica napus, cDNAs encoding Delta(1)-pyrroline-5-carboxylate synthetase (BnP5CS), ornithine delta-aminotransferase (BnOAT) and proline dehydrogenase (BnPDH) were isolated and characterized. Southern blot analysis of BnP5CSs in B. napus and its diploid ancestors suggested a gene loss may have occurred during evolution. The expression of BnP5CS1 and BnP5CS2 was induced, while the expression of BnPDH was inhibited under salt stress, ABA treatment and dehydration, prior to proline accumulation. The upregulation of BnOAT expression was only detected during prolonged severe osmotic stress. Our results indicate that stress-induced proline accumulation in B. napus results from the reciprocal action of activated biosynthesis and inhibited proline degradation. Whether the ornithine pathway is activated depends on the severity of stress. During development, proline content was high in reproductive organs and was accompanied by markedly high expression of BnP5CS and BnPDH, suggesting possible roles of proline during flower development. [BMB reports 2009; 42(1): 28-34].
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1976-6696
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28-34
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Proline accumulation and transcriptional regulation of proline biosynthesis and degradation in Brassica napus.
pubmed:affiliation
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't