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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1991-11-21
|
| pubmed:abstractText |
The 323/A3 murine monoclonal antibody, initially described as reactive to breast carcinomas, is found by immunohistological analyses to have broad cross reactivity with adenocarcinomas of diverse histologic origin. The 323/A3 antigen is similar to the tumor-associated 17-1A antigen as revealed by immunoblot and cross-competition cell binding studies. We have investigated the potential use of the 323/A3 monoclonal antibody for tumor imaging as a Fab' molecule labeled with 99mTc. In vitro studies demonstrate that 323/A3 Fab' has high affinity (2-3 x 10(9) M-1) with no significant loss of immunoreactivity compared to the intact IgG. In vivo studies demonstrate that 99mTc 323/A3 Fab' can rapidly detect human breast and colon tumor xenografts growing in athymic nude mice. Distinct breast tumor visualization is observed as early as 1 h post intravenous administration with the 99mTc 323/A3 Fab'. Distinct colon tumor visualization is observed by 3 h (the earliest time point imaged). Tumor-to-blood ratios are higher for 99mTc 323/A3 Fab' than with a 99mTc-labeled nonspecific isotype-matched Fab' antibody. These results suggest that 99mTc 323/A3 Fab' can detect 17-1A antigen and may have potential clinical utility for the rapid diagnostic imaging of adenocarcinomas.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0883-2897
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
18
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
483-97
|
| pubmed:dateRevised |
2008-2-21
|
| pubmed:meshHeading |
pubmed-meshheading:1917518-Adenocarcinoma,
pubmed-meshheading:1917518-Animals,
pubmed-meshheading:1917518-Antibodies, Monoclonal,
pubmed-meshheading:1917518-Blotting, Northern,
pubmed-meshheading:1917518-Breast Neoplasms,
pubmed-meshheading:1917518-Colonic Neoplasms,
pubmed-meshheading:1917518-Evaluation Studies as Topic,
pubmed-meshheading:1917518-Female,
pubmed-meshheading:1917518-Humans,
pubmed-meshheading:1917518-Hybridomas,
pubmed-meshheading:1917518-Immunoglobulin Fab Fragments,
pubmed-meshheading:1917518-Immunohistochemistry,
pubmed-meshheading:1917518-Isotope Labeling,
pubmed-meshheading:1917518-Mice,
pubmed-meshheading:1917518-Mice, Nude,
pubmed-meshheading:1917518-Peptide Fragments,
pubmed-meshheading:1917518-Technetium,
pubmed-meshheading:1917518-Tumor Cells, Cultured
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Evaluation of the 323/A3 monoclonal antibody and the use of technetium-99m-labeled 323/A3 Fab' for the detection of pan adenocarcinoma.
|
| pubmed:affiliation |
Research and Development Division, Centocor, Inc., Malvern, PA 19355.
|
| pubmed:publicationType |
Journal Article
|