Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1991-11-21
|
| pubmed:abstractText |
Diploid human fibroblasts were transfected with a plasmid carrying a v-myc oncogene linked to the neo gene or with a vector control carrying a neo gene. Drug-resistant clones were isolated and subcultured as needed. All populations went into crisis and eventually senesced. But while they were senescing, viable-appearing clones were noted among the progeny of a transfected population that expressed the v-myc oncogene. After several months, these cells began replicating more rapidly. Karyotype analysis indicated that they were clonally derived since all of them had 45 chromosomes, including 2 marker chromosomes. This cell strain was designated MSU-1.1. Similar analysis showed that cells from an earlier passage were diploid. These cells were designated MSU-1.0. Both strains have undergone more than 200 population doublings since their siblings senesced, without any change in chromosome complement. Both strains express the v-myc protein and have the same integration site for the transfected v-myc and neo genes. The MSU-1.0 cells cannot grow without exogenously added growth factors. The MSU-1.1 cells grow moderately well under the same conditions and grow to a higher saturation density than MSU-1.0 cells. Since the chance of human cells acquiring an infinite life span in culture is very rare, the data suggest that MSU-1.1 cells are derived from MSU-1.0 cells. The expression of v-myc is probably required for acquisition of an infinite life span, since this phenotype did not develop in populations not expressing this oncogene. However, expression of v-myc is clearly not sufficient, since all of the progeny of the clone that gave rise to the MSU-1.0 cells expressed this oncogene, but the vast majority of them senesced.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0014-4827
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
197
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
125-36
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1915659-Animals,
pubmed-meshheading:1915659-Blotting, Southern,
pubmed-meshheading:1915659-Cell Death,
pubmed-meshheading:1915659-Cell Division,
pubmed-meshheading:1915659-Cell Line, Transformed,
pubmed-meshheading:1915659-Chickens,
pubmed-meshheading:1915659-Chromosome Banding,
pubmed-meshheading:1915659-Clone Cells,
pubmed-meshheading:1915659-Culture Media, Serum-Free,
pubmed-meshheading:1915659-Diploidy,
pubmed-meshheading:1915659-Fibroblasts,
pubmed-meshheading:1915659-Genes, myc,
pubmed-meshheading:1915659-Growth Substances,
pubmed-meshheading:1915659-Humans,
pubmed-meshheading:1915659-Karyotyping,
pubmed-meshheading:1915659-Male,
pubmed-meshheading:1915659-Mice,
pubmed-meshheading:1915659-Neoplasm Transplantation,
pubmed-meshheading:1915659-Time Factors,
pubmed-meshheading:1915659-Transfection
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Characteristics of an infinite life span diploid human fibroblast cell strain and a near-diploid strain arising from a clone of cells expressing a transfected v-myc oncogene.
|
| pubmed:affiliation |
Department of Microbiology, Michigan State University, East Lansing 48824.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|