Source:http://linkedlifedata.com/resource/pubmed/id/19143227
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2009-1-15
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| pubmed:abstractText |
The effects of insoluble dietary fibre differing in lignin content on intestinal morphology and mRNA expression was tested in an animal model of 48 weaned piglets. Engaged fibre sources were wheat bran (rich in cellulose and hemicellulose) and pollen from Chinese Masson pine (Pinus massoniana) (rich in lignin), respectively. The fibre sources were added to a basal diet as follows: no addition (control), 3.0% wheat bran, 1.27% pine pollen, and 2.55% pine pollen. The 12 animals of each feeding group were fed four experimental diets ad libitum for 37 days and were then slaughtered for retrieving tissue samples from stomach, jejunum, ileum, colon and mesenterial lymph nodes. Both fibre sources increased villus height of mucosa in jejunum (+10% on average) and ileum (+16% on average). Results of mRNA expression rates of inflammatory, cell cycle and growth marker genes (NFkappaB, TNFalpha, TGFbeta, Caspase3, CDK4, IGF1) were specific to fibre source and tissue: wheat bran induced an up-regulation of NFkappaB in stomach and jejunum, as well as TNFalpha and TGFbeta, and Caspase3 in jejunum. Pine pollen induced down regulation of NFkappaB, TNFalpha, TGFbeta, Caspase3, CDK4 and IGF1 in the colon as well as up-regulation of NFkappaB and TGFbeta in mesenterial lymph nodes. Finally, an overall data comparison based on a hierarchical cluster analysis showed a close relation between gene regulation in different gut sections and organs, as well as between small intestine morphology and zootechnical performance.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
1745-039X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
62
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
427-38
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| pubmed:meshHeading |
pubmed-meshheading:19143227-Animal Feed,
pubmed-meshheading:19143227-Animal Nutritional Physiological Phenomena,
pubmed-meshheading:19143227-Animals,
pubmed-meshheading:19143227-Animals, Newborn,
pubmed-meshheading:19143227-Cluster Analysis,
pubmed-meshheading:19143227-Dietary Fiber,
pubmed-meshheading:19143227-Disease Models, Animal,
pubmed-meshheading:19143227-Dose-Response Relationship, Drug,
pubmed-meshheading:19143227-Female,
pubmed-meshheading:19143227-Gene Expression Regulation,
pubmed-meshheading:19143227-Intestinal Mucosa,
pubmed-meshheading:19143227-Lignin,
pubmed-meshheading:19143227-Male,
pubmed-meshheading:19143227-RNA, Messenger,
pubmed-meshheading:19143227-Random Allocation,
pubmed-meshheading:19143227-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19143227-Solubility,
pubmed-meshheading:19143227-Swine,
pubmed-meshheading:19143227-Weaning
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| pubmed:year |
2008
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| pubmed:articleTitle |
Effect of insoluble fibre on intestinal morphology and mRNA expression pattern of inflammatory, cell cycle and growth marker genes in a piglet model.
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| pubmed:affiliation |
University of Natural Resources and Applied Life Sciences, Vienna, Austria.
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| pubmed:publicationType |
Journal Article
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