Linked Life Data

19142909

Source:http://linkedlifedata.com/resource/pubmed/id/19142909

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-2-2
pubmed:abstractText
This review introduces to the readers our new perspectives of polymer-based monolithic column with a high performance for small solutes such as drug candidates, illustrating the fabrication of LC columns in capillary. First, we briefly reviewed the status quo of polymer-based monolithic columns, comparing with silica monoliths. The miniaturization of LC system with higher throughput (shorter analytical time) was stressed conceptually, along with a fine permeable bicontinuous monolithic structure with submicron domain size (skeletal thickness + pore size) for higher performance. Second, from these perspectives, our column preparation was described, while our specially designed porogenic solvents were introduced as a controller of the monolithic morphology via reaction-induced phase separation. Specifically, monolithic columns were exemplified in two polymer formats, that is, one monolith prepared by free radical polymerization of glycerin 1,3-dimethacrylate, GDMA, and the other prepared by stepwise polymerization of newly introduced multifunctional epoxy and diamino monomers. Both monolithic columns in capillary format demonstrated a fine bicontinuous structure, affording a good compatibility of the efficiency (H) and permeability (D). Especially, the epoxy-based column showed an excellent separation impedance, E (=H(2)/D). Our micro-HPLC data were discussed along with a prototyped wired chip device.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Feb
pubmed:issn
1615-9314
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
341-58
pubmed:year
2009
pubmed:articleTitle
Polymer-based monolithic columns in capillary format tailored by using controlled in situ polymerization.
pubmed:affiliation
Division of Applied Science for Functionality, Graduate School of Science & Technology, Kyoto Institute of Technology, Kyoto, Japan. hjn62658@mtb.biglobe.ne.jp
pubmed:publicationType
Journal Article