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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-11-14
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pubmed:abstractText |
The simultaneous detection of anti-La, anti-60-kD Ro and anti-52-kD Ro antibodies by immunoblotting is greatly improved by changing the crosslinking level in the gel to an acrylamide/bisacrylamide ratio of 19:1. Using this method for the analysis of a number of systemic lupus erythematosus (SLE) and Sjögren's syndrome patient sera it was observed that antibody to the 52-kD Ro protein without anti-60-kD Ro antibody was restricted to Sjögren's syndrome patients (9/26), whereas antibody to the 60-kD Ro protein without contaminating anti-52-kD Ro antibody was only found in SLE patients (8/38). Moreover, in Sjögren's syndrome patient sera anti-Ro antibody was found only in combination with anti-La antibody (20/26), whereas in SLE patient sera anti-Ro antibody could be found without detectable anti-La specificity (4/38). Double immunofluorescence microscopy revealed that the 52-kD Ro and the 60-kD Ro proteins co-localize in the cytoplasm as well as in the nucleus, whereas immunoprecipitation of [32P]-labelled HeLa cell extract with monospecific anti-52-kD Ro and anti-60-kD Ro sera showed that both proteins are associated with the Ro RNAs. These data suggest the presence of both the 52-kD and the 60-kD Ro proteins in the same ribonucleoprotein complexes. To study the evolutionary conservation of the 52-kD Ro, the 60-kD Ro and the La proteins, extracts of cell lines derived from various mammalian species were analysed on Western blots using monospecific human antibodies. In contrast to the 60-kD Ro and the La antigens which are well conserved in evolution, the 52-kD Ro antigen could be detected in primate cells only by this immunological approach.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-1690756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-1709666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-1716477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2107806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2113988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2194109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2332496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2351837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2414294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2468131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2484307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2503280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2506878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2597211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2649513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-2784800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-3062767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-3200833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-3367095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-3884199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-6172778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-6180298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-6357777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-6421292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-6712685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-6804494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-6866113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-7097683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-7138600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-7276162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1914239-7406938
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
99-105
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1914239-Antibody Specificity,
pubmed-meshheading:1914239-Autoantibodies,
pubmed-meshheading:1914239-Autoantigens,
pubmed-meshheading:1914239-Autoimmune Diseases,
pubmed-meshheading:1914239-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1914239-Humans,
pubmed-meshheading:1914239-Molecular Weight,
pubmed-meshheading:1914239-Nuclear Proteins,
pubmed-meshheading:1914239-RNA, Small Cytoplasmic,
pubmed-meshheading:1914239-Ribonucleoproteins,
pubmed-meshheading:1914239-Species Specificity,
pubmed-meshheading:1914239-Tumor Cells, Cultured
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pubmed:year |
1991
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pubmed:articleTitle |
Detection and occurrence of the 60- and 52-kD Ro (SS-A) antigens and of autoantibodies against these proteins.
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pubmed:affiliation |
Department of Biochemistry, University of Nijmegen, The Netherlands.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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