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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2009-2-6
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pubmed:abstractText |
Dehydroepiandrosterone (DHEA) is used as a dietary supplement and can be metabolized to androgens and/or estrogens in the prostate. We investigated the hypothesis that DHEA metabolism may be increased in a reactive prostate stroma environment in the presence of proinflammatory cytokines such as transforming growth factor beta1 (TGFbeta1), and further, whether red clover extract, which contains a variety of compounds including isoflavones, can reverse this effect. LAPC-4 prostate cancer cells were grown in coculture with prostate stromal cells (6S) and treated with DHEA +/- TGFbeta1 or interleukin-6. Prostate-specific antigen (PSA) expression and testosterone secretion in LAPC-4/6S cocultures were compared with those in monocultured epithelial and stromal cells by real-time PCR and/or ELISA. Combined administration of TGFbeta1 + DHEA to cocultures increased PSA protein secretion two to four times, and PSA gene expression up to 50-fold. DHEA + TGFbeta1 also increased coculture production of testosterone over DHEA treatment alone. Red clover isoflavone treatment led to a dose-dependent decrease in PSA protein and gene expression and testosterone metabolism induced by TGFbeta1 + DHEA in prostate LAPC-4/6S cocultures. In this coculture model of endocrine-immune-paracrine interactions in the prostate, TGFbeta1 greatly increased stromal-mediated DHEA effects on testosterone production and epithelial cell PSA production, whereas red clover isoflavones reversed these effects.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19141600-10037394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19141600-10084567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19141600-10373668,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19141600-17384581,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19141600-9168795
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1940-6215
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
134-42
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pubmed:dateRevised |
2010-9-23
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pubmed:meshHeading |
pubmed-meshheading:19141600-Adjuvants, Immunologic,
pubmed-meshheading:19141600-Cells, Cultured,
pubmed-meshheading:19141600-Coculture Techniques,
pubmed-meshheading:19141600-Dehydroepiandrosterone,
pubmed-meshheading:19141600-Endocrine System,
pubmed-meshheading:19141600-Epithelial Cells,
pubmed-meshheading:19141600-Eugenia,
pubmed-meshheading:19141600-Extracellular Matrix Proteins,
pubmed-meshheading:19141600-Humans,
pubmed-meshheading:19141600-Interleukin-6,
pubmed-meshheading:19141600-Male,
pubmed-meshheading:19141600-Paracrine Communication,
pubmed-meshheading:19141600-Phytotherapy,
pubmed-meshheading:19141600-Plant Extracts,
pubmed-meshheading:19141600-Prostate,
pubmed-meshheading:19141600-Prostate-Specific Antigen,
pubmed-meshheading:19141600-Prostatic Neoplasms,
pubmed-meshheading:19141600-Stromal Cells,
pubmed-meshheading:19141600-Testosterone,
pubmed-meshheading:19141600-Transforming Growth Factor beta
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pubmed:year |
2009
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pubmed:articleTitle |
Endocrine-immune-paracrine interactions in prostate cells as targeted by phytomedicines.
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pubmed:affiliation |
Endocrine Section, Laboratory of Clinical Investigation, Division of Intramural Research, National Center for Complementary and Alternative Medicine, NIH, Bethesda, Maryland, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Intramural
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