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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2009-2-2
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pubmed:abstractText |
CpG-DNA is currently attracting attention as an effective and safe vaccine adjuvant to prevent from microbial infections. In this report, we examined the effects of oligo B, which is a synthetic CpG-DNA, in mucosal administration of Bacillus Calmette-Guérin (BCG) and diphtheria toxoid (DT). Co-administration with oligo B enhanced BCG-induced delayed type hypersensitivity to purified protein derivative (PPD) in guinea pigs. The titers of anti-DT serum IgG, IgA and mucosal IgA antibodies induced by intranasal administration with DT plus oligo B were significantly higher than that with DT alone. In both C57BL/6 and BALB/c mice, intranasal administration of DT with oligo B induced enough level of antibodies to prevent onset of diphtheria. The analysis of antibody subclasses showed that intranasal administration of oligo B induced not only IgG1 but also IgG2a, IgG2c and IgA anti-DT antibodies. In contrast, there was no or little production of the anti-DT serum IgE. Taken together our data suggest that oligo B is a powerful adjuvant in mucosal immunization.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/CPG-oligonucleotide,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Diphtheria Toxoid,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin A,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1166-73
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pubmed:meshHeading |
pubmed-meshheading:19136040-Adjuvants, Immunologic,
pubmed-meshheading:19136040-Administration, Intranasal,
pubmed-meshheading:19136040-Animals,
pubmed-meshheading:19136040-Antibodies, Bacterial,
pubmed-meshheading:19136040-Cytokines,
pubmed-meshheading:19136040-Diphtheria Toxoid,
pubmed-meshheading:19136040-Female,
pubmed-meshheading:19136040-Guinea Pigs,
pubmed-meshheading:19136040-Immunity, Mucosal,
pubmed-meshheading:19136040-Immunoglobulin A,
pubmed-meshheading:19136040-Immunoglobulin E,
pubmed-meshheading:19136040-Immunoglobulin G,
pubmed-meshheading:19136040-Leukocytes, Mononuclear,
pubmed-meshheading:19136040-Mice,
pubmed-meshheading:19136040-Mice, Inbred BALB C,
pubmed-meshheading:19136040-Mice, Inbred C57BL,
pubmed-meshheading:19136040-Mucous Membrane,
pubmed-meshheading:19136040-Mycobacterium bovis,
pubmed-meshheading:19136040-Oligodeoxyribonucleotides,
pubmed-meshheading:19136040-Spleen,
pubmed-meshheading:19136040-Tuberculin Test
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pubmed:year |
2009
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pubmed:articleTitle |
The mucosal adjuvanticity of the oligodeoxynucleotides containing a non-methylated CpG motif on BCG and diphtheria toxoid.
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pubmed:affiliation |
Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Gakuen, Musashimurayama, Tokyo 208-0011, Japan. meymj@nih.go.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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