Source:http://linkedlifedata.com/resource/pubmed/id/19130397
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2009-1-8
|
| pubmed:abstractText |
The mechanism of transmission of amyloid protein, especially the dynamics in the intestine, is still largely unknown. In the present study, a fusion protein (Abeta-EGFP) that combined enhanced green fluorescent protein with amyloid-beta protein (Abeta) was orally administered to mice before and after weaning, and the uptake and kinetics of amyloid protein within the intestine were elucidated through histopathology. Abeta-EGFP was incorporated into the cytoplasm of columnar epithelial cells, rather than M cells, at 3 h after administration and thereafter. Abeta-EGFP then accumulated in the crypt, Peyer's patch, and even the spleen. However, this uptake was not observed in weaned mice. These results suggest that a specific tolerant mechanism for incorporation of Abeta escaped from the digestion exists during suckling periods. This age-dependent uptake is important for estimating the risk of transmission.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1699-5848
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:volume |
24
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
283-92
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:19130397-Administration, Oral,
pubmed-meshheading:19130397-Amyloid beta-Peptides,
pubmed-meshheading:19130397-Animals,
pubmed-meshheading:19130397-Animals, Suckling,
pubmed-meshheading:19130397-Epithelium,
pubmed-meshheading:19130397-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:19130397-Green Fluorescent Proteins,
pubmed-meshheading:19130397-Immunohistochemistry,
pubmed-meshheading:19130397-Intestinal Mucosa,
pubmed-meshheading:19130397-Kinetics,
pubmed-meshheading:19130397-Mice,
pubmed-meshheading:19130397-Mice, Inbred ICR,
pubmed-meshheading:19130397-Microvilli,
pubmed-meshheading:19130397-Models, Biological,
pubmed-meshheading:19130397-Peyer's Patches,
pubmed-meshheading:19130397-Protein Structure, Secondary,
pubmed-meshheading:19130397-Recombinant Fusion Proteins,
pubmed-meshheading:19130397-Spectroscopy, Fourier Transform Infrared,
pubmed-meshheading:19130397-Spleen,
pubmed-meshheading:19130397-Time Factors,
pubmed-meshheading:19130397-Tissue Distribution
|
| pubmed:year |
2009
|
| pubmed:articleTitle |
Intestinal uptake of amyloid beta protein through columnar epithelial cells in suckling mice.
|
| pubmed:affiliation |
Department of Molecular Immunology, Graduate School of Agricultural and Life Sciences, the University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|