Linked Life Data

19130216

Source:http://linkedlifedata.com/resource/pubmed/id/19130216

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-4-28
pubmed:abstractText
Neural stem cells (NSC) with self-renewal and multipotent properties could provide an ideal cell source for transplantation to treat spinal cord injury, stroke, and neurodegenerative diseases. However, the majority of transplanted NSC and neural progenitor cells (NPC) differentiate into astrocytes in vivo under pathological environments in the central nervous system, which potentially cause reactive gliosis. Because the serum is a potent inducer of astrocyte differentiation of rodent NPC in culture, we studied the effect of the serum on gene expression profile of cultured human NPC to identify the gene signature of astrocyte differentiation of human NPC. Human NPC spheres maintained in the serum-free culture medium were exposed to 10% fetal bovine serum (FBS) for 72 h, and processed for analyzing on a Whole Human Genome Microarray of 41,000 genes, and the microarray data were validated by real-time RT-PCR. The serum elevated the levels of expression of 45 genes, including ID1, ID2, ID3, CTGF, TGFA, METRN, GFAP, CRYAB and CSPG3, whereas it reduced the expression of 23 genes, such as DLL1, DLL3, PDGFRA, SOX4, CSPG4, GAS1 and HES5. Thus, the serum-induced astrocyte differentiation of human NPC is characterized by a counteraction of ID family genes on Delta family genes. Coimmunoprecipitation analysis identified ID1 as a direct binding partner of a proneural basic helix-loop-helix (bHLH) transcription factor MASH1. Luciferase assay indicated that activation of the DLL1 promoter by MASH1 was counteracted by ID1. Bone morphogenetic protein 4 (BMP4) elevated the levels of ID1 and GFAP expression in NPC under the serum-free culture conditions. Because the serum contains BMP4, these results suggest that the serum factor(s), most probably BMP4, induces astrocyte differentiation by upregulating the expression of ID family genes that repress the proneural bHLH protein-mediated Delta expression in human NPC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1573-6830
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
423-38
pubmed:meshHeading
pubmed-meshheading:19130216-Astrocytes, pubmed-meshheading:19130216-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:19130216-Bone Morphogenetic Protein 4, pubmed-meshheading:19130216-Cell Differentiation, pubmed-meshheading:19130216-Cells, Cultured, pubmed-meshheading:19130216-Down-Regulation, pubmed-meshheading:19130216-Female, pubmed-meshheading:19130216-Gene Expression Profiling, pubmed-meshheading:19130216-Gene Expression Regulation, Developmental, pubmed-meshheading:19130216-Glial Fibrillary Acidic Protein, pubmed-meshheading:19130216-Humans, pubmed-meshheading:19130216-Infant, pubmed-meshheading:19130216-Inhibitor of Differentiation Protein 1, pubmed-meshheading:19130216-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:19130216-Intermediate Filament Proteins, pubmed-meshheading:19130216-Models, Biological, pubmed-meshheading:19130216-Nerve Tissue Proteins, pubmed-meshheading:19130216-Neurons, pubmed-meshheading:19130216-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:19130216-Promoter Regions, Genetic, pubmed-meshheading:19130216-Reproducibility of Results, pubmed-meshheading:19130216-Serum, pubmed-meshheading:19130216-Stem Cells, pubmed-meshheading:19130216-Up-Regulation
pubmed:year
2009
pubmed:articleTitle
Gene expression profiling of human neural progenitor cells following the serum-induced astrocyte differentiation.
pubmed:affiliation
Department of Bioinformatics and Molecular Neuropathology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo, 204-8588, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't