| pubmed-article:19121345 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19121345 | lifeskim:mentions | umls-concept:C0030274 | lld:lifeskim |
| pubmed-article:19121345 | lifeskim:mentions | umls-concept:C1801960 | lld:lifeskim |
| pubmed-article:19121345 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:19121345 | lifeskim:mentions | umls-concept:C0699733 | lld:lifeskim |
| pubmed-article:19121345 | lifeskim:mentions | umls-concept:C0681828 | lld:lifeskim |
| pubmed-article:19121345 | lifeskim:mentions | umls-concept:C1708375 | lld:lifeskim |
| pubmed-article:19121345 | lifeskim:mentions | umls-concept:C0137909 | lld:lifeskim |
| pubmed-article:19121345 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:19121345 | pubmed:dateCreated | 2009-2-3 | lld:pubmed |
| pubmed-article:19121345 | pubmed:abstractText | A system for vascular hollow fiber bio-artificial pancreas development, optimization and in vitro testing was implemented and operated in a simple and fully described manner, allowing other researchers to test a variety of experimental conditions (different biomaterials, biologic tissue, addition of proteins or other adjuvants). In this work, a polysulfone hollow fiber was used as bioprotective material. Two different cell sources were co-immobilized with agarose microspheres in and experimented with the membrane device: rat islets of Langerhans and mouse beta-TC-3 insulinoma cells. The results obtained with islets of Langerhans were used as islet comparable insulin-release data. Beta-TC-3 cells were mainly used in these studies, due to higher control and reproducibility of cell number and behavior: addition of hemoglobin was beneficial for sustained cell viability, especially during cell insertion in the device (viability assessed by beta-TC-3 lactate dehydrogenase activity in the recirculating culture medium); cells did not adhere to the polysulfone membrane (assessed by SEM observation of membrane samples from dynamic cultures). Comparable device functionality and insulin-release results were attained with both cell types: device functionality was maintained for 7-9 days and maximum insulin-release during dynamic glucose challenges were 2.6 x 10(-3)+/-7 x 10(-5)microU/beta-cell x 8 h, with islets, and 9.3 x 10(-4)+/-2 x 10(-5)microU/beta-cell x 2 h, with beta-TC-3 cells. | lld:pubmed |
| pubmed-article:19121345 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19121345 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19121345 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19121345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19121345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19121345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19121345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19121345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19121345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19121345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19121345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19121345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19121345 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19121345 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:19121345 | pubmed:issn | 0168-1656 | lld:pubmed |
| pubmed-article:19121345 | pubmed:author | pubmed-author:SilviA AAA | lld:pubmed |
| pubmed-article:19121345 | pubmed:author | pubmed-author:MateusMM | lld:pubmed |
| pubmed-article:19121345 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:19121345 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:19121345 | pubmed:volume | 139 | lld:pubmed |
| pubmed-article:19121345 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19121345 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19121345 | pubmed:pagination | 236-49 | lld:pubmed |
| pubmed-article:19121345 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:19121345 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19121345 | pubmed:articleTitle | Development of a polysulfone hollow fiber vascular bio-artificial pancreas device for in vitro studies. | lld:pubmed |
| pubmed-article:19121345 | pubmed:affiliation | Institute of Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, Instituto Superior Técnico, Lisboa, Portugal. | lld:pubmed |
| pubmed-article:19121345 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19121345 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19121345 | lld:pubmed |
