Source:http://linkedlifedata.com/resource/pubmed/id/19121345
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2009-2-3
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| pubmed:abstractText |
A system for vascular hollow fiber bio-artificial pancreas development, optimization and in vitro testing was implemented and operated in a simple and fully described manner, allowing other researchers to test a variety of experimental conditions (different biomaterials, biologic tissue, addition of proteins or other adjuvants). In this work, a polysulfone hollow fiber was used as bioprotective material. Two different cell sources were co-immobilized with agarose microspheres in and experimented with the membrane device: rat islets of Langerhans and mouse beta-TC-3 insulinoma cells. The results obtained with islets of Langerhans were used as islet comparable insulin-release data. Beta-TC-3 cells were mainly used in these studies, due to higher control and reproducibility of cell number and behavior: addition of hemoglobin was beneficial for sustained cell viability, especially during cell insertion in the device (viability assessed by beta-TC-3 lactate dehydrogenase activity in the recirculating culture medium); cells did not adhere to the polysulfone membrane (assessed by SEM observation of membrane samples from dynamic cultures). Comparable device functionality and insulin-release results were attained with both cell types: device functionality was maintained for 7-9 days and maximum insulin-release during dynamic glucose challenges were 2.6 x 10(-3)+/-7 x 10(-5)microU/beta-cell x 8 h, with islets, and 9.3 x 10(-4)+/-2 x 10(-5)microU/beta-cell x 2 h, with beta-TC-3 cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Hemoglobins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/L-Lactate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Membranes, Artificial,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfones,
http://linkedlifedata.com/resource/pubmed/chemical/polysulfone P 1700
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0168-1656
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
139
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
236-49
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:19121345-Animals,
pubmed-meshheading:19121345-Bioartificial Organs,
pubmed-meshheading:19121345-Cell Adhesion,
pubmed-meshheading:19121345-Cell Line, Tumor,
pubmed-meshheading:19121345-Cell Survival,
pubmed-meshheading:19121345-Cells, Cultured,
pubmed-meshheading:19121345-Cells, Immobilized,
pubmed-meshheading:19121345-Culture Media,
pubmed-meshheading:19121345-Equipment Design,
pubmed-meshheading:19121345-Glucose,
pubmed-meshheading:19121345-Hemoglobins,
pubmed-meshheading:19121345-Insulin,
pubmed-meshheading:19121345-Islets of Langerhans,
pubmed-meshheading:19121345-L-Lactate Dehydrogenase,
pubmed-meshheading:19121345-Membranes, Artificial,
pubmed-meshheading:19121345-Mice,
pubmed-meshheading:19121345-Models, Biological,
pubmed-meshheading:19121345-Pancreas, Artificial,
pubmed-meshheading:19121345-Polymers,
pubmed-meshheading:19121345-Rats,
pubmed-meshheading:19121345-Sulfones
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| pubmed:year |
2009
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| pubmed:articleTitle |
Development of a polysulfone hollow fiber vascular bio-artificial pancreas device for in vitro studies.
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| pubmed:affiliation |
Institute of Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, Instituto Superior Técnico, Lisboa, Portugal.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|