Linked Life Data

19107412

Source:http://linkedlifedata.com/resource/pubmed/id/19107412

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-12-24
pubmed:abstractText
Ni-NTA affinity chromatography under denaturing conditions has proven to be a powerful method for the isolation of SUMO conjugates from total cell extracts, as it minimizes deconjugation and excludes noncovalent interactions. This chapter describes the use of both His-tagged SUMO and a His-tagged target protein for the characterization of the sumoylation process in the budding yeast Saccharomyces cerevisiae. Two well-studied model substrates, the septin Cdc3 and the replication clamp protein PCNA, are used as examples, but the protocol can easily be adapted to other targets and organisms.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
497
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-103
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
In vivo detection and characterization of sumoylation targets in Saccharomyces cerevisiae.
pubmed:affiliation
Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, UK.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't