Linked Life Data

19100876

Source:http://linkedlifedata.com/resource/pubmed/id/19100876

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-12-22
pubmed:abstractText
Rhodamine B hydroxylamide (1) is characterized as a highly selective and sensitive fluorescence probe for Cu(2+). Under the optimized conditions, the probe exhibits specific absorbance-on and fluorescence-on responses to Cu(2+) only. This remarkable property may allow Cu(2+) to be detected directly in the presence of the other transition metal ions, and such an application has been demonstrated to human serum. The reaction mechanism is also investigated and proposed as that the hydroxylamide group of 1 binds Cu(2+), and the subsequent complexation of Cu(2+) displays a high catalytic activity for the hydrolytic cleavage of the amide bond, causing the release of fluorophore (rhodamine B) and thereby the retrievement of absorbance and fluorescence. The recovered fluorescence intensity is proportional to the concentration of Cu(2+) in the range 1-20 microM. The detection limit for Cu(2+) is 33 nM (k=3). The reaction mechanism described here may be useful for developing excellent spectroscopic probes with cleavable active bonds for other analytes.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Jan
pubmed:issn
1873-4324
pubmed:author
pubmed:issnType
Electronic
pubmed:day
19
pubmed:volume
632
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9-14
pubmed:year
2009
pubmed:articleTitle
Characterization of rhodamine B hydroxylamide as a highly selective and sensitive fluorescence probe for copper(II).
pubmed:affiliation
Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
pubmed:publicationType
Journal Article