|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2009-12-16
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Taenia solium Linnaeus, 1758 is responsible for taeniasis and cysticercosis, which are 2 serious health problems, particularly in developing countries. The attempt to identify a 22.5kD possible protective oncospheral antigen by 2-dimensional gel-electrophoresis, micro-sequencing, and cDNA library screening produced a protein of 42kD that possesses a conserved domain similar to that of troponin T. Five variants that showed differences at the 5' end were observed at the cDNA level. Hyper-immune rabbit sera developed against recombinant GST fused protein identified the protein exclusively on activated oncospheres. The 42kD protein was tested in an enzyme-linked immunoassay (ELISA) alone and then together with the Tso31 protein for the diagnosis of human cysticercosis. When both antigens were combined, the test was found to be 85% sensitive and 65% specific. The 42kD is a novel T. solium protein that is present exclusively on activated oncospheres of this parasite, with poor diagnostic activity against taeniasis or human cysticercosis.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
1937-2345
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
95
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
591-7
|
|
pubmed:meshHeading |
pubmed-meshheading:19090648-Amino Acid Sequence,
pubmed-meshheading:19090648-Animals,
pubmed-meshheading:19090648-Base Sequence,
pubmed-meshheading:19090648-Cysticercosis,
pubmed-meshheading:19090648-DNA, Complementary,
pubmed-meshheading:19090648-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:19090648-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:19090648-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:19090648-Helminth Proteins,
pubmed-meshheading:19090648-Humans,
pubmed-meshheading:19090648-Molecular Sequence Data,
pubmed-meshheading:19090648-Polymerase Chain Reaction,
pubmed-meshheading:19090648-Rabbits,
pubmed-meshheading:19090648-Recombinant Proteins,
pubmed-meshheading:19090648-Sensitivity and Specificity,
pubmed-meshheading:19090648-Sequence Analysis, Protein,
pubmed-meshheading:19090648-Taenia solium,
pubmed-meshheading:19090648-Taeniasis,
pubmed-meshheading:19090648-Troponin T
|
|
pubmed:year |
2009
|
|
pubmed:articleTitle |
A novel Taenia solium protein that resembles troponin T proteins.
|
|
pubmed:affiliation |
Departamento de Microbiologia, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porres, Lima 31, Peru.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
