Source:http://linkedlifedata.com/resource/pubmed/id/19084575
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2009-2-2
|
| pubmed:databankReference | |
| pubmed:abstractText |
Multiple forms of 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD) and their differential tissue expression pattern have not been shown in any lower vertebrates. In the present study, we report cloning of two novel 3beta-HSDs and two variants from gonads of the Nile tilapia. 3beta-HSD cDNAs encode two peptides of 375 (3beta-HSD type-I/variant 1) and 367 (3beta-HSD type-II/variant 1) amino acid residues that share 31.9% homology. 3beta-HSD type-I/variant 1 shared high homology with other piscine counterparts while 3beta-HSD type-II/variant 1 exhibited homology to mammalian DeltaC27-3beta-HSD and multifunctional viral 3beta-HSD. The latter seems to be ancient form among vertebrates. Transiently transfected 3beta-HSDs' open reading frames in COS-7 cells converted exogenous pregnenolone/androsta-5-ene-3beta-17beta-diol to progesterone/testosterone. Tissue distribution pattern of 3beta-HSDs by RT-PCR revealed varied expression pattern. Northern blot analysis of 3beta-HSDs demonstrated steady or gradual rise in transcripts level at different gonadal stages. These data revealed the importance of novel 3beta-HSDs in teleosts and also provided phylogenetic significance.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0303-7207
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
27
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| pubmed:volume |
299
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
146-52
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| pubmed:meshHeading |
pubmed-meshheading:19084575-17-Hydroxysteroid Dehydrogenases,
pubmed-meshheading:19084575-Amino Acid Sequence,
pubmed-meshheading:19084575-Animals,
pubmed-meshheading:19084575-Blotting, Northern,
pubmed-meshheading:19084575-COS Cells,
pubmed-meshheading:19084575-Cercopithecus aethiops,
pubmed-meshheading:19084575-Cichlids,
pubmed-meshheading:19084575-Cloning, Molecular,
pubmed-meshheading:19084575-Female,
pubmed-meshheading:19084575-Gene Expression Profiling,
pubmed-meshheading:19084575-Isoenzymes,
pubmed-meshheading:19084575-Male,
pubmed-meshheading:19084575-Molecular Sequence Data,
pubmed-meshheading:19084575-Ovary,
pubmed-meshheading:19084575-Phylogeny,
pubmed-meshheading:19084575-Reproduction,
pubmed-meshheading:19084575-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19084575-Testis,
pubmed-meshheading:19084575-Transfection
|
| pubmed:year |
2009
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| pubmed:articleTitle |
Novel 3beta-hydroxysteroid dehydrogenases from gonads of the Nile tilapia: phylogenetic significance and expression during reproductive cycle.
|
| pubmed:affiliation |
Laboratory of Reproductive Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan. bsksl@uohyd.ernet.in
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|