Linked Life Data

19077008

Source:http://linkedlifedata.com/resource/pubmed/id/19077008

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-3-12
pubmed:abstractText
Although viable fungi have been recovered from a wide variety of icy environments, their metabolic capabilities under frozen conditions are still largely unknown. We investigated basidiomycetous yeasts isolated from an Antarctic ice core and showed that after freezing at a relatively slow rate (0.8 degrees C min(-1)), the cells are excluded into veins of liquid at the triple junctions of ice crystals. These strains were capable of reproductive growth at -5 degrees C under liquid conditions. Under frozen conditions, metabolic activity was assessed by measuring rates of [(3)H]leucine incorporation into the acid-insoluble macromolecular fraction, which decreased exponentially at temperatures between 15 degrees C and -15 degrees C and was inhibited by the protein synthesis inhibitor cycloheximide. Experiments at -5 degrees C under frozen and liquid conditions revealed 2-3 orders of magnitude lower rates of endogenous metabolism in ice, likely due to the high salinity in the liquid fraction of the ice (equivalent of approximately 1.4 mol l(-1) of NaCl at -5 degrees C). The mesophile Saccharomyces cerevisae also incorporated [(3)H]leucine at -5 degrees C and -15 degrees C, indicating that this activity is not exclusive to cold-adapted microorganisms. The ability of yeast cells to incorporate amino acid substrates into macromolecules and remain metabolically active under these conditions has implications for understanding the survival of Eukarya in icy environments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1462-2920
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
589-96
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Macromolecular synthesis by yeasts under frozen conditions.
pubmed:affiliation
Department of Biological Sciences, Louisiana State University, Life Sciences Building, Baton Rouge, LA 70803, USA. pamato1@lsu.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't