Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-1-29
pubmed:abstractText
Toxin-antitoxin (TA) loci are common in free-living bacteria and archaea. TA loci encode a stable toxin that is neutralized by a metabolically unstable antitoxin. The antitoxin can be either a protein or an antisense RNA. So far, six different TA gene families, in which the antitoxins are proteins, have been identified. Recently, Makarova et al. (K. S. Makarova, N. V. Grishin, and E. V. Koonin, Bioinformatics 22:2581-2584, 2006) suggested that the hicAB loci constitute a novel TA gene family. Using the hicAB locus of Escherichia coli K-12 as a model system, we present evidence that supports this inference: expression of the small HicA protein (58 amino acids [aa]) induced cleavage in three model mRNAs and tmRNA. Concomitantly, the global rate of translation was severely reduced. Using tmRNA as a substrate, we show that HicA-induced cleavage does not require the target RNA to be translated. Expression of HicB (145 aa) prevented HicA-mediated inhibition of cell growth. These results suggest that HicB neutralizes HicA and therefore functions as an antitoxin. As with other antitoxins (RelB and MazF), HicB could resuscitate cells inhibited by HicA, indicating that ectopic production of HicA induces a bacteriostatic rather than a bactericidal condition. Nutrient starvation induced strong hicAB transcription that depended on Lon protease. Mining of 218 prokaryotic genomes revealed that hicAB loci are abundant in bacteria and archaea.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-10508174, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-10829079, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-11273701, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-11454201, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-11717402, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-12010492, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-12526800, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-12603745, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-12787364, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-12972253, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-1316444, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-1324324, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-14580342, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-14659018, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-15228536, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-15576765, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-15718296, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-15768033, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-15864262, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-16109374, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-16257530, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-16621839, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-16672603, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-16740961, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-16768798, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-16895922, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-17172327, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-17263853, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-17291332, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-17367382, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-17369330, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-17513477, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-17706670, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-17962566, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-18191220, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-18398006, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-18757857, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-18974181, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-2691330, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-7508988, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-7608087, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-8226627, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-8650219, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-9721313, http://linkedlifedata.com/resource/pubmed/commentcorrection/19060138-9767574
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1098-5530
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
191
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1191-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
HicA of Escherichia coli defines a novel family of translation-independent mRNA interferases in bacteria and archaea.
pubmed:affiliation
Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle NE2 4HH, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't