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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1977-4-30
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pubmed:abstractText |
The method employed to determine the sequence of a T1 RNase fragment, A-A-A-A-A-U-A-A-C-A-A-U-A-C-A-Gp, from Novikoff rat hepatoma 18S ribosomal RNA is described. This method is applicable to any oligoribonucleotide produced by specific endonucleases that leave the newly cleaved 5'-end free for labeling with polynucleotide kinase and gamma-(32p)-ATP. The (32p)-labeled oligoribonucleotide is subjected to partial endonucleolytic digestion and fractionated by two-dimensional homochromatography fingerprinting. The nucleotide sequence is determined by following mobility shifts of the labeled and partially digested oligoribonucleotides in homochromatography fingerprinting.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-1055375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-1263259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-13018277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-170097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-173855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-188015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-4335584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-4362631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-5318919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-5323016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-5340184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-5401237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-5639916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/190590-776961
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
339-52
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:190590-Animals,
pubmed-meshheading:190590-Base Sequence,
pubmed-meshheading:190590-Carcinoma, Hepatocellular,
pubmed-meshheading:190590-Liver Neoplasms,
pubmed-meshheading:190590-Methods,
pubmed-meshheading:190590-Neoplasms, Experimental,
pubmed-meshheading:190590-Oligoribonucleotides,
pubmed-meshheading:190590-RNA, Ribosomal,
pubmed-meshheading:190590-Rats,
pubmed-meshheading:190590-Ribonuclease T1,
pubmed-meshheading:190590-Ribonucleases
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pubmed:year |
1977
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pubmed:articleTitle |
Sequence analysis of T1 ribonuclease fragments of 18S ribosomal RNA by 5'-terminal labeling, partial digestion, and homochromatography fingerprinting.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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