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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1991-7-5
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pubmed:abstractText |
Alpha complementation of beta-galactosidase (beta gal) is intracistronic and requires interaction between the alpha donor region (residues 3-41) and alpha acceptor fragment (produced by M15). We have constructed two plasmids which direct the synthesis of hybrid beta gal: coxsackievirus proteins in Escherichia coli. One plasmid, pBD1045, encodes an enzymatically active 3C protease of coxsackievirus B3 fused between the amino-terminal 79 amino acids of beta gal (containing the alpha donor region) and amino acids 80 to 1023 (alpha acceptor region). A second plasmid, pBD1043 encodes an inactive 3C protease and results in a fusion of 260 coxsackievirus amino acids between residues 79 and 80 of the beta gal monomer. Both hybrid proteins expressed by these constructs have beta-galactosidase activity regardless of whether the viral protease (183 amino acids) is autocatalytically cleaved out of the chimeric protein (pBD1045) or remains as part of a fusion protein (pBD1043). The implications of these results for structural flexibility of the complemented beta-galactosidase enzyme are discussed.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3C proteases,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
243-51
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pubmed:dateRevised |
2007-10-11
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pubmed:meshHeading |
pubmed-meshheading:1904218-Base Sequence,
pubmed-meshheading:1904218-Blotting, Western,
pubmed-meshheading:1904218-Chimera,
pubmed-meshheading:1904218-Cysteine Endopeptidases,
pubmed-meshheading:1904218-Enterovirus,
pubmed-meshheading:1904218-Escherichia coli,
pubmed-meshheading:1904218-Genes, Bacterial,
pubmed-meshheading:1904218-Kinetics,
pubmed-meshheading:1904218-Molecular Sequence Data,
pubmed-meshheading:1904218-Plasmids,
pubmed-meshheading:1904218-Recombinant Fusion Proteins,
pubmed-meshheading:1904218-Restriction Mapping,
pubmed-meshheading:1904218-Viral Proteins,
pubmed-meshheading:1904218-beta-Galactosidase
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pubmed:year |
1991
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pubmed:articleTitle |
Expression of functional beta-galactosidase containing the coxsackievirus 3C protease as an internal fusion.
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pubmed:affiliation |
Schering-Plough Research, Bloomfield, New Jersey 07003.
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pubmed:publicationType |
Journal Article
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