|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2008-12-23
|
|
pubmed:abstractText |
Suppressor of cytokine signaling-3 (SOCS-3) plays an important role in negative regulation of inflammatory response. Evidence has shown that SOCS-3 and IL-10 expressions were significantly reduced in placental trophoblasts from preeclampsia. IL-10 is an anti-inflammatory cytokine. In this study, we sought to determine if enhance SOCS-3 expression could affect IL-10 production in placental trophoblasts. Placental JEG-3 cells were used. Over-expression of SOCS-3 was generated by transfection of JEG-3 cells with a green fluorescent protein (GFP) tagged SOCS-3 gene, SOCS-3/ZsGreen1, by siPORT lipid transfection. Cells transfected with ZsGreen1 vector only was used as control. Our results showed that IL-6 production was reduced in cells over-expressed with SOCS-3. Moreover, SOCS-3 transfected cells produced more IL-10 when stimulated with IL-6. The increased IL-10 production by JEG-3 cells was in a dose-dependent manner, p<0.05. Our data suggested that enhanced SOCS-3 gene expression could promote IL-10 production by placental trophoblast cells, suggesting that SOCS-3 may play an important role in regulation of cytokine induced anti-inflammatory response in placental trophoblasts.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-10477622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-10490099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-10490100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-11481489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-11907070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-12431386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-12754506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-12967647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-16040151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-16352613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-16712928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-16816341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-17215490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-1827484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-18986700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-2454191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-3501121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-7593621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-7722286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19036437-8402911
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/IL10 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/IL6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/SOCS3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Suppressor of Cytokine Signaling...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0143-4004
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
30
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
11-4
|
|
pubmed:dateRevised |
2011-7-27
|
|
pubmed:meshHeading |
pubmed-meshheading:19036437-Cell Line, Tumor,
pubmed-meshheading:19036437-Choriocarcinoma,
pubmed-meshheading:19036437-Culture Media, Conditioned,
pubmed-meshheading:19036437-Dose-Response Relationship, Drug,
pubmed-meshheading:19036437-Female,
pubmed-meshheading:19036437-Gene Expression Regulation, Developmental,
pubmed-meshheading:19036437-Green Fluorescent Proteins,
pubmed-meshheading:19036437-Humans,
pubmed-meshheading:19036437-Interleukin-10,
pubmed-meshheading:19036437-Interleukin-6,
pubmed-meshheading:19036437-Signal Transduction,
pubmed-meshheading:19036437-Suppressor of Cytokine Signaling Proteins,
pubmed-meshheading:19036437-Transfection,
pubmed-meshheading:19036437-Trophoblasts,
pubmed-meshheading:19036437-Uterine Neoplasms
|
|
pubmed:year |
2009
|
|
pubmed:articleTitle |
Over-expression of SOCS-3 gene promotes IL-10 production by JEG-3 trophoblast cells.
|
|
pubmed:affiliation |
Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|
