Linked Life Data

19020106

Source:http://linkedlifedata.com/resource/pubmed/id/19020106

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-1-5
pubmed:abstractText
The dengue virus (DENV) has four distinct serotypes (DENV1, DENV2, DENV3, and DENV4) that require differentiation for effective prevention of morbid diseases. The recently developed DENV1-specific NS1 antigen capture enzyme-linked immunosorbent assay (ELISA) based on the monoclonal antibodies (MAbs) that recognize distinct epitopes on nonstructural protein 1 (NS1) of a specific DENV serotype is convenient and cost-effective, but assays have not yet been developed for DENV serotypes 2 to 4. This paper describes the development and validation of a DENV2-specific NS1 antigen capture ELISA by selection and optimization of the pair of well-characterized MAbs that recognized epitopes specific for DENV2 NS1 from a large panel of MAbs. The DENV2 NS1 ELISA displayed exclusive sensitivity with the DENV2 serotype and did not cross-react with the other three DENV serotypes. The sensitivity and specificity of the DENV2 NS1 ELISA were 83.3% (25/30) and 100% (504/504) when used to test 30 acute-phase serum samples from patients infected with DENV2 identified by virus isolation or reverse transcription-PCR serotyping and 504 serum samples from healthy individuals, respectively. The specificity of this assay was also evaluated using a panel of serum samples which were positive for DENV1, other flaviviruses, and nonflaviviruses; no cross-reactions were observed in these clinical samples. The DENV2 NS1 ELISA was eightfold more sensitive than a commercially available serotype-cross-reactive NS1 ELISA (Panbio Diagnostics, Brisbane, Australia) when the two assays were used to test the DENV2-infected cell culture supernatants in parallel. The Panbio NS1 ELISA displayed variation in sensitivity between DENV serotypes. The DENV2-specific NS1 antigen capture ELISA can be used as a tool for the rapid identification of DENV2 infections.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-10698995, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-11596093, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-11825945, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-11892494, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-11980973, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-12624024, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-12820947, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-12951221, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-12958240, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-1372617, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-14718083, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-15184444, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-15577938, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-15672450, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-15897988, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-1617166, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-16524419, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-16873905, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-16891505, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-16988003, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-17140671, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-17409201, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-17558424, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-17653311, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-17889487, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-18476771, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-1849979, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-18685015, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-2150838, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-2434611, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-2525840, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-3510163, http://linkedlifedata.com/resource/pubmed/commentcorrection/19020106-9665979
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1556-679X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
88-95
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Development of an antigen capture immunoassay based on monoclonal antibodies specific for dengue virus serotype 2 nonstructural protein 1 for early and rapid identification of dengue virus serotype 2 infections.
pubmed:affiliation
Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Validation Studies