Source:http://linkedlifedata.com/resource/pubmed/id/19006119
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2008-12-29
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| pubmed:abstractText |
Interleukin-6 (IL-6) is involved in a variety of biological responses, including the glucose metabolism and cell growth, which is a critical physiological function requiring multiple metabolic pathways. Therefore, in the present study, we examined the effect of IL-6 on 2-deoxyglucose (2-DG) uptake and the related signaling pathways in primary cultured chicken hepatocytes. IL-6 increased 2-DG uptake in a time- (> or =4 h) and a dose -(> or =5 ng/ml) dependent manner. Indeed, IL-6 increased GLUT-2 mRNA and protein expression as well as 2-DG uptake, which were blocked by actinomycin D (AD, transcription inhibitor) and cycloheximide (CHX, translation inhibitor). IL-6 (10 ng/ml) increased the level of IL-6Ralpha and glycoprotein (gp) 130 (IL-6Rbeta) protein expressions. IL-6 increased Janus Kinase (JAK)-2, signal transducer and activator of transcription (STAT)-3 phosphorylation, intracellular Ca(2+) concentration, and PKC phosphorylation. IL-6-induced increase of 2-DG uptake and GLUT-2 protein expression were blocked by JAK2-specific siRNA, a STAT3 inhibitor, staurosporine, and bisindolylmaleimide I (PKC inhibitors). In addition, IL-6 increased EGFR/src/FAK, PI3K/Akt phosphorylation and 2-DG uptake as well as GLUT-2 protein expression, which were blocked by AG 1478 (EGF receptor inhibitor), PP2 (src family of tyrosine kinase inhibitor), PI3K-specific siRNA, and a Akt inhibitor. Furthermore, IL-6 increased p44/42 MAPKs phosphorylation and p44 and p42 MAPK-specific siRNA mixture blocked IL-6-induced increase of 2-DG uptake and GLUT-2 protein expression. In conclusion, IL-6 stimulates the 2-DG uptake through p44/42 MAPKs activation via Ca(2+)/PKC and EGF receptor in primary cultured chicken hepatocytes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyglucose,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/STAT Transcription Factors
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1097-4652
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
218
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
643-52
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:19006119-Animals,
pubmed-meshheading:19006119-Calcium,
pubmed-meshheading:19006119-Cells, Cultured,
pubmed-meshheading:19006119-Chickens,
pubmed-meshheading:19006119-Deoxyglucose,
pubmed-meshheading:19006119-Enzyme Activation,
pubmed-meshheading:19006119-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:19006119-Hepatocytes,
pubmed-meshheading:19006119-Interleukin-6,
pubmed-meshheading:19006119-Janus Kinases,
pubmed-meshheading:19006119-Male,
pubmed-meshheading:19006119-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:19006119-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:19006119-Models, Biological,
pubmed-meshheading:19006119-Protein Kinase C,
pubmed-meshheading:19006119-Receptor, Epidermal Growth Factor,
pubmed-meshheading:19006119-Receptors, Interleukin-6,
pubmed-meshheading:19006119-STAT Transcription Factors
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| pubmed:year |
2009
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| pubmed:articleTitle |
Interleukin-6 promotes 2-deoxyglucose uptake through p44/42 MAPKs activation via Ca2+/PKC and EGF receptor in primary cultured chicken hepatocytes.
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| pubmed:affiliation |
Department of Veterinary Physiology, Biotherapy Human Resources Center (BK21), College of Veterinary Medicine, Chonnam National University, Gwangju, South Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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