Linked Life Data

1900249

Source:http://linkedlifedata.com/resource/pubmed/id/1900249

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-4-8
pubmed:databankReference
pubmed:abstractText
The product of the Saccharomyces cerevisiae AER2 gene is responsible for maintaining repression of at least two distinct regulatory pathways: heme activation/repression and catabolite repression. Mutations in the gene caused an eightfold increase in the expression of the heme-activated CYC1 gene in the absence of heme, a substantial increase in the expression of the heme-repressed ANB1 gene in the presence of heme, and a 13-fold increase in the expression of the catabolite-repressed GAL1 gene in the presence of glucose. Lesser or no increases in the expression of these genes were observed under derepressed or activation conditions. The aer2 mutations also caused a large increase in CYC7 gene expression under all conditions; this gene is subject to heme activation/repression, as well as catabolite repression. The AER2 gene was cloned and the sequence determined. The large open reading frame contiguous with the transcript from the complementing region encoded a 713-amino acid polypeptide chain with extensive homology to the beta-subunit of G proteins. The sequence revealed that AER2 is the TUP1 gene. A deletion mutation was constructed and the null phenotype was the same as the original mutants. The aer2 null mutant was shown to have increased aerobic and anaerobic levels of RNA encoding the ROX1 repressor, normally expressed only aerobically and responsible for the aerobic repression of ANB1 expression. The increase in both ROX1 and ANB1 RNAs aerobically in this mutant suggests that the repressor is nonfunctional in the mutant.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
97
pubmed:geneSymbol
AER2, CYC1, ROX1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1900249-Amino Acid Sequence, pubmed-meshheading:1900249-Base Sequence, pubmed-meshheading:1900249-Chromosome Mapping, pubmed-meshheading:1900249-Chromosomes, Fungal, pubmed-meshheading:1900249-Cloning, Molecular, pubmed-meshheading:1900249-Fungal Proteins, pubmed-meshheading:1900249-GTP-Binding Proteins, pubmed-meshheading:1900249-Gene Expression Regulation, Fungal, pubmed-meshheading:1900249-Genes, Fungal, pubmed-meshheading:1900249-Genes, Regulator, pubmed-meshheading:1900249-Heme, pubmed-meshheading:1900249-Molecular Sequence Data, pubmed-meshheading:1900249-Mutation, pubmed-meshheading:1900249-Phenotype, pubmed-meshheading:1900249-Repressor Proteins, pubmed-meshheading:1900249-Restriction Mapping, pubmed-meshheading:1900249-Saccharomyces cerevisiae, pubmed-meshheading:1900249-Sequence Homology, Nucleic Acid, pubmed-meshheading:1900249-Transcription, Genetic
pubmed:year
1991
pubmed:articleTitle
A yeast protein with homology to the beta-subunit of G proteins is involved in control of heme-regulated and catabolite-repressed genes.
pubmed:affiliation
Department of Biological Sciences, State University of New York, Albany 12222.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.