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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2009-2-20
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pubmed:abstractText |
Understanding the pathways that regulate the human T-cell acute lymphoblastic leukemia (T-ALL) initiating cells (T-LiC) activity has been hampered by the lack of biologic assays in which this human disease can be studied. Here we show that coculture of primary human T-ALL with a mouse stromal cell line expressing the NOTCH ligand delta-like-1 (DL1) reproducibly allowed maintenance of T-LiC and long-term growth of blast cells. Human T-ALL mutated or not on the NOTCH receptor required sustained activation of the NOTCH pathway via receptor/ligand interaction for growth and T-LiC activity. On the reverse, inhibition of the NOTCH pathway during primary cultures abolished in vitro cell growth and in vivo T-LiC activity. Altogether, these results demonstrate the major role of the NOTCH pathway activation in human T-ALL development and in the maintenance of leukemia-initiating cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amyloid Precursor Protein Secretases,
http://linkedlifedata.com/resource/pubmed/chemical/DLK1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NOTCH1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Notch1,
http://linkedlifedata.com/resource/pubmed/chemical/benzyloxycarbonyl-leucyl-leucyl-norl...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1528-0020
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pubmed:author |
pubmed-author:ArmstrongFlorenceF,
pubmed-author:BalleriniPaolaP,
pubmed-author:BaruchelAndréA,
pubmed-author:BoisselNicolasN,
pubmed-author:Brunet de la GrangePhilippeP,
pubmed-author:CalvoJulienJ,
pubmed-author:DombretHervéH,
pubmed-author:FontenayMichaélaM,
pubmed-author:GerbyBastienB,
pubmed-author:Landman-ParkerJudithJ,
pubmed-author:PflumioFrançoiseF,
pubmed-author:RoméoPaul-HenriPH,
pubmed-author:RouyezMarie-ChristineMC
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pubmed:issnType |
Electronic
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pubmed:day |
19
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pubmed:volume |
113
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1730-40
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pubmed:dateRevised |
2009-6-26
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pubmed:meshHeading |
pubmed-meshheading:18984862-Amyloid Precursor Protein Secretases,
pubmed-meshheading:18984862-Animals,
pubmed-meshheading:18984862-Cell Communication,
pubmed-meshheading:18984862-Cell Culture Techniques,
pubmed-meshheading:18984862-Coculture Techniques,
pubmed-meshheading:18984862-Genes, T-Cell Receptor gamma,
pubmed-meshheading:18984862-Humans,
pubmed-meshheading:18984862-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:18984862-Membrane Proteins,
pubmed-meshheading:18984862-Mice,
pubmed-meshheading:18984862-Mice, Inbred NOD,
pubmed-meshheading:18984862-Mice, SCID,
pubmed-meshheading:18984862-Neoplasm Transplantation,
pubmed-meshheading:18984862-Oligopeptides,
pubmed-meshheading:18984862-Precursor T-Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:18984862-Receptor, Notch1,
pubmed-meshheading:18984862-Signal Transduction,
pubmed-meshheading:18984862-Stromal Cells,
pubmed-meshheading:18984862-Tumor Cells, Cultured
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pubmed:year |
2009
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pubmed:articleTitle |
NOTCH is a key regulator of human T-cell acute leukemia initiating cell activity.
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pubmed:affiliation |
Institut Cochin, Université Paris Descartes, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 8104 Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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