|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2008-11-28
|
|
pubmed:abstractText |
We examined the effect of celecoxib on the expression of T-cell factors (TCFs) to clarify the mechanism by which celecoxib suppress beta-catenin/TCF-dependent transcriptional activity without reducing the level of beta-catenin protein, using HCT-116 cells. Celecoxib suppressed the expression of TCF-1 and TCF-4 in a time-dependent manner. Pretreatment of cells with the proteasome inhibitor MG132 inhibited the loss of TCF-1 and TCF-4 induced by celecoxib, suggesting that celecoxib induced the proteasome-dependent degradation of TCF-1 and TCF-4. Beta-catenin/TCF-dependent transcriptional activity was significantly decreased after the treatment with celecoxib for 6 h and the pretreatment of the cells with MG132 attenuated the effect of celecoxib. Further, celecoxib also suppressed the expression of TCF-1 and TCF-4 in another colon cancer cell line, DLD-1. Our results suggest that TCF-1 and TCF-4 degradation may involve the inhibition of the Wnt/beta-catenin signaling pathway induced by celecoxib.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents...,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Proteinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Leupeptins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteasome Endopeptidase Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides,
http://linkedlifedata.com/resource/pubmed/chemical/T Cell Transcription Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/TCF Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/TCF7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TCF7L2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor 7-Like 2...,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/benzyloxycarbonylleucyl-leucyl-leuci...,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/celecoxib
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
1090-2104
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
26
|
|
pubmed:volume |
377
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1185-90
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:18983819-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:18983819-Cell Line, Tumor,
pubmed-meshheading:18983819-Colonic Neoplasms,
pubmed-meshheading:18983819-Cysteine Proteinase Inhibitors,
pubmed-meshheading:18983819-Humans,
pubmed-meshheading:18983819-Leupeptins,
pubmed-meshheading:18983819-Proteasome Endopeptidase Complex,
pubmed-meshheading:18983819-Pyrazoles,
pubmed-meshheading:18983819-Sulfonamides,
pubmed-meshheading:18983819-T Cell Transcription Factor 1,
pubmed-meshheading:18983819-TCF Transcription Factors,
pubmed-meshheading:18983819-Transcription, Genetic,
pubmed-meshheading:18983819-Transcription Factor 7-Like 2 Protein,
pubmed-meshheading:18983819-Wnt Proteins,
pubmed-meshheading:18983819-beta Catenin
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Celecoxib-induced degradation of T-cell factors-1 and -4 in human colon cancer cells.
|
|
pubmed:affiliation |
Department of Clinical Pharmacology, Faculty of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan. yanaga@clipharm.med.kyushu-u.ac.jp
|
|
pubmed:publicationType |
Journal Article
|
