Linked Life Data

18981247

Source:http://linkedlifedata.com/resource/pubmed/id/18981247

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-12-22
pubmed:databankReference
pubmed:abstractText
Five types of cytolethal distending toxin (CDT-I to CDT-V) have been identified in Escherichia coli. In the present study we cloned and sequenced the cdt-IV operon and flanking region from a porcine extraintestinal pathogenic E. coli (ExPEC) strain belonging to serogroup O75. We confirmed that similar to other CDTs, CDT-IV induced phosphorylation of host histone H2AX, a sensitive marker of DNA double-strand breaks, and blocked the HeLa cell cycle at the G(2)-M transition. The cdt-IV genes were framed by lambdoid prophage genes. We cloned and sequenced the cdt-I operon and flanking regions from a human ExPEC O18:K1:H7 strain and observed that cdt-I genes were also flanked by lambdoid prophage genes. PCR studies indicated that a gene coding for a putative protease was always associated with the cdtC-IV gene but was not associated with cdtC genes in strains producing CDT-I, CDT-III, and CDT-V. Our results suggest that the cdt-I and cdt-IV genes might have been acquired from a common ancestor by phage transduction and evolved in their bacterial hosts. The lysogenic bacteriophages have the potential to carry nonessential "cargo" genes or "morons" and therefore play a crucial role in the generation of genetic diversity within ExPEC.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-10473386, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-10798561, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-10972814, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-11030657, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-11083828, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-11179379, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-11206551, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-11453636, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-11500438, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-11526041, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-11597449, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-11896765, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-12496208, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-12761152, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-12933829, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-12947116, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-12958258, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-12969375, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-14623014, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-14660372, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-14742559, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-14977993, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-15056215, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-15070766, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-15164065, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-15260895, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-15353570, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-15694861, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-15737728, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-16861635, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-16902142, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-17293413, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-17601779, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-17726095, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-2580792, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-3447015, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-372481, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-7984417, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-8112838, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-8170993, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-8262635, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-9220015, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-9436272, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-9508292, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981247-9712806
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1098-5522
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
492-500
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Cytolethal distending toxin type I and type IV genes are framed with lambdoid prophage genes in extraintestinal pathogenic Escherichia coli.
pubmed:affiliation
Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary. Tothi@vmri.hu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't