| pubmed-article:18972511 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18972511 | lifeskim:mentions | umls-concept:C0335038 | lld:lifeskim |
| pubmed-article:18972511 | lifeskim:mentions | umls-concept:C0011923 | lld:lifeskim |
| pubmed-article:18972511 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:18972511 | lifeskim:mentions | umls-concept:C0598496 | lld:lifeskim |
| pubmed-article:18972511 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:18972511 | lifeskim:mentions | umls-concept:C1521840 | lld:lifeskim |
| pubmed-article:18972511 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:18972511 | pubmed:dateCreated | 2008-11-4 | lld:pubmed |
| pubmed-article:18972511 | pubmed:abstractText | Noninvasive imaging of specific mRNAs in living subjects promises numerous biological and medical applications. Common strategies use fluorescently or radioactively labelled antisense probes to detect target mRNAs through a hybridization mechanism, but have met with limited success in living animals. Here we present a novel molecular imaging approach based on the group I intron of Tetrahymena thermophila for imaging mRNA molecules in vivo. Engineered trans-splicing ribozyme reporters contain three domains, each of which is designed for targeting, splicing, and reporting. They can transduce the target mRNA into a reporter mRNA, leading to the production of reporter enzymes that can be noninvasively imaged in vivo. We have demonstrated this ribozyme-mediated RNA imaging method for imaging a mutant p53 mRNA both in single cells and noninvasively in living mice. After optimization, the ribozyme reporter increases contrast for the transiently expressed target by 180-fold, and by ten-fold for the stably expressed target. siRNA-mediated specific gene silencing of p53 expression has been successfully imaged in real time in vivo. This new ribozyme-based RNA reporter system should open up new avenues for in vivo RNA imaging and direct imaging of siRNA inhibition. | lld:pubmed |
| pubmed-article:18972511 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18972511 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18972511 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18972511 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18972511 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18972511 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18972511 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18972511 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18972511 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18972511 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18972511 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18972511 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18972511 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:18972511 | pubmed:issn | 1439-7633 | lld:pubmed |
| pubmed-article:18972511 | pubmed:author | pubmed-author:HasegawaSumit... | lld:pubmed |
| pubmed-article:18972511 | pubmed:author | pubmed-author:ChungJune-Key... | lld:pubmed |
| pubmed-article:18972511 | pubmed:author | pubmed-author:RaoJianghongJ | lld:pubmed |
| pubmed-article:18972511 | pubmed:author | pubmed-author:GowrishankarG... | lld:pubmed |
| pubmed-article:18972511 | pubmed:author | pubmed-author:SoMin-KyungMK | lld:pubmed |
| pubmed-article:18972511 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18972511 | pubmed:day | 3 | lld:pubmed |
| pubmed-article:18972511 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:18972511 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18972511 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18972511 | pubmed:pagination | 2682-91 | lld:pubmed |
| pubmed-article:18972511 | pubmed:dateRevised | 2010-4-29 | lld:pubmed |
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| pubmed-article:18972511 | pubmed:meshHeading | pubmed-meshheading:18972511... | lld:pubmed |
| pubmed-article:18972511 | pubmed:meshHeading | pubmed-meshheading:18972511... | lld:pubmed |
| pubmed-article:18972511 | pubmed:meshHeading | pubmed-meshheading:18972511... | lld:pubmed |
| pubmed-article:18972511 | pubmed:meshHeading | pubmed-meshheading:18972511... | lld:pubmed |
| pubmed-article:18972511 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18972511 | pubmed:articleTitle | Imaging target mRNA and siRNA-mediated gene silencing in vivo with ribozyme-based reporters. | lld:pubmed |
| pubmed-article:18972511 | pubmed:affiliation | Molecular Imaging Program at Stanford, Department of Radiology, Biophysics, Cancer Biology Programs, Stanford University School of Medicine, 1201 Welch Road, Stanford, California 94305-5484, USA. | lld:pubmed |
| pubmed-article:18972511 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18972511 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:18972511 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:18972511 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
