Source:http://linkedlifedata.com/resource/pubmed/id/18972511
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
16
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| pubmed:dateCreated |
2008-11-4
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| pubmed:abstractText |
Noninvasive imaging of specific mRNAs in living subjects promises numerous biological and medical applications. Common strategies use fluorescently or radioactively labelled antisense probes to detect target mRNAs through a hybridization mechanism, but have met with limited success in living animals. Here we present a novel molecular imaging approach based on the group I intron of Tetrahymena thermophila for imaging mRNA molecules in vivo. Engineered trans-splicing ribozyme reporters contain three domains, each of which is designed for targeting, splicing, and reporting. They can transduce the target mRNA into a reporter mRNA, leading to the production of reporter enzymes that can be noninvasively imaged in vivo. We have demonstrated this ribozyme-mediated RNA imaging method for imaging a mutant p53 mRNA both in single cells and noninvasively in living mice. After optimization, the ribozyme reporter increases contrast for the transiently expressed target by 180-fold, and by ten-fold for the stably expressed target. siRNA-mediated specific gene silencing of p53 expression has been successfully imaged in real time in vivo. This new ribozyme-based RNA reporter system should open up new avenues for in vivo RNA imaging and direct imaging of siRNA inhibition.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Catalytic,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Lactamases
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
1439-7633
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
3
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| pubmed:volume |
9
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2682-91
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| pubmed:dateRevised |
2010-4-29
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| pubmed:meshHeading |
pubmed-meshheading:18972511-Animals,
pubmed-meshheading:18972511-Base Sequence,
pubmed-meshheading:18972511-COS Cells,
pubmed-meshheading:18972511-Cercopithecus aethiops,
pubmed-meshheading:18972511-Exons,
pubmed-meshheading:18972511-Gene Silencing,
pubmed-meshheading:18972511-Genes, Reporter,
pubmed-meshheading:18972511-Genes, p53,
pubmed-meshheading:18972511-Humans,
pubmed-meshheading:18972511-Luciferases,
pubmed-meshheading:18972511-Mice,
pubmed-meshheading:18972511-Mutation,
pubmed-meshheading:18972511-Protein Biosynthesis,
pubmed-meshheading:18972511-RNA, Catalytic,
pubmed-meshheading:18972511-RNA, Messenger,
pubmed-meshheading:18972511-RNA, Small Interfering,
pubmed-meshheading:18972511-RNA Stability,
pubmed-meshheading:18972511-Tetrahymena thermophila,
pubmed-meshheading:18972511-Time Factors,
pubmed-meshheading:18972511-beta-Lactamases
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| pubmed:year |
2008
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| pubmed:articleTitle |
Imaging target mRNA and siRNA-mediated gene silencing in vivo with ribozyme-based reporters.
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| pubmed:affiliation |
Molecular Imaging Program at Stanford, Department of Radiology, Biophysics, Cancer Biology Programs, Stanford University School of Medicine, 1201 Welch Road, Stanford, California 94305-5484, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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