Source:http://linkedlifedata.com/resource/pubmed/id/18966828
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2008-10-30
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| pubmed:abstractText |
A novel amperometric biosensor for the determination of lactate was constructed by first immobilizing lactate oxidase and an osmium redox polymer ([Os(bpy)(2)(PVP)(10)Cl]Cl; abbreviated Os-polymer) on the surface of a glassy carbon electrode, followed by coating with a sol-gel film derived from methyltriethoxysilane (MTEOS). The electrooxidation current of this electrode was found to be diffusion controlled. In the presence of lactate, a clear electrocatalytic oxidation wave was observed, and lactate could be determined amperometrically at 400 mV versus Ag AgCl . The concentration range of linear response, slope of linear response and detection limit were 0.1-9 mM, 1.02 microA mM(-1), and 0.05 mM, respectively. Although L-ascorbate was electrooxidized at this potential, uric acid, paracetamol and glucose were found not to interfere.
|
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0039-9140
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
973-8
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| pubmed:year |
1997
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| pubmed:articleTitle |
Sol-gel based amperometric biosensor incorporating an osmium redox polymer as mediator for detection of L-lactate.
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| pubmed:affiliation |
BEST Centre, School of Chemical Sciences, Dubin City University, Dublin 9, Ireland.
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| pubmed:publicationType |
Journal Article
|